Prevalence and antifungal susceptibility of Candida albicans and its related species Candida dubliniensis and Candida africana isolated from vulvovaginal samples in a hospital of Argentina
| Autor: | Emilce Méndez, Soledad Gamarra, Guillermo Garcia-Effron, Laura Theill, Susana Morano, María Elena Nardin, Catiana Dudiuk |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Resistencia a los antifúngicos Microbiology (medical) Antifungal Agents Itraconazole 030106 microbiology Argentina Microbial Sensitivity Tests Microbiology Vulva 03 medical and health sciences Candidiasis vulvovaginal Amphotericin B Candida albicans medicine Humans Candidiasis Vulvovaginal Candida Voriconazole biology Vulvovaginal candidiasis Candida africana General Medicine Antifungal resistance biology.organism_classification Corpus albicans Nystatin Identificación molecular Vagina Female Molecular identification Fluconazole Candida dubliniensis medicine.drug |
| Zdroj: | Revista Argentina de Microbiología. 48(1):43-49 |
| ISSN: | 0325-7541 |
| DOI: | 10.1016/j.ram.2015.10.003 |
| Popis: | Candida africana taxonomical status is controversial. It was proposed as a separate species within the Candida albicans species complex; however, phylogenetic analyses suggested that it is an unusual variety of C. albicans. The prevalence of C. albicans-related species (Candida dubliniensis and C. africana) as vulvovaginal pathogens is not known in Argentina. Moreover, data on antifungal susceptibility of isolates causing vulvovaginal candidiasis is scarce. The aims of this study were to establish the prevalence of C. dubliniensis and C. africana in vaginal samples and to evaluate the antifungal susceptibilities of vaginal C. albicans species complex strains. We used a molecular-based method coupled with a new pooled DNA extraction methodology to differentiate C. dubliniensis and C. africana in a collection of 287 strains originally identified as C. albicans isolated from an Argentinian hospital during 2013. Antifungal susceptibilities to fluconazole, clotrimazole, itraconazole, voriconazole, nystatin, amphotericin B and terbinafine were evaluated by using the CLSI M27-A3 and M27-S4 documents. Of the 287 isolates, 4 C. dubliniensis and one C. africana strains (1.39% and 0.35% prevalence, respectively) were identified. This is the first description of C. africana in Argentina and its identification was confirmed by sequencing the ITS2 region and the hwp1 gene. C. dubliniensis and C. africana strains showed very low MIC values for all the tested antifungals. Fluconazole-reduced-susceptibility and azole cross-resistance were observed in 3.55% and 1.41% of the C. albicans isolates, respectively. These results demonstrate that antifungal resistance is still a rare phenomenon in this kind of isolates. |
| Databáze: | OpenAIRE |
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