CD8(+)CD25(+) T cells reduce atherosclerosis in apoE(-/-) mice
| Autor: | Portia Trinidad, Xiaoning Zhao, Juliana Yano, Paul C. Dimayuga, Jianchang Zhou, Prediman K. Shah, Tomoyuki Honjo, Kuang-Yuh Chyu, Wai Man Lio, Bojan Cercek |
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| Rok vydání: | 2013 |
| Předmět: |
Apolipoprotein E
Male Adoptive cell transfer Biophysics Intracellular Space chemical and pharmacologic phenomena Biology CD8-Positive T-Lymphocytes Biochemistry Interleukin 21 Mice Apolipoproteins E medicine Cytotoxic T cell Animals IL-2 receptor Molecular Biology Cell Proliferation Interleukin-2 Receptor alpha Subunit Cell Biology Arteriosclerosis medicine.disease Atherosclerosis Molecular biology Adoptive Transfer In vitro Plaque Atherosclerotic Diet Mice Inbred C57BL Phenotype Immunology CD8 Biomarkers Spleen |
| Zdroj: | Biochemical and biophysical research communications. 443(3) |
| ISSN: | 1090-2104 |
| Popis: | Background It is increasingly evident that CD8+ T cells are involved in atherosclerosis but the specific subtypes have yet to be defined. CD8+CD25+ T cells exert suppressive effects on immune signaling and modulate experimental autoimmune disorders but their role in atherosclerosis remains to be determined. The phenotype and functional role of CD8+CD25+ T cells in experimental atherosclerosis were investigated in this study. Methods and results CD8+CD25+ T cells were observed in atherosclerotic plaques of apoE(−/−) mice fed hypercholesterolemic diet. Characterization by flow cytometric analysis and functional evaluation using a CFSE-based proliferation assays revealed a suppressive phenotype and function of splenic CD8+CD25+ T cells from apoE(−/−) mice. Depletion of CD8+CD25+ from total CD8+ T cells rendered higher cytolytic activity of the remaining CD8+CD25− T cells. Adoptive transfer of CD8+CD25+ T cells into apoE(−/−) mice suppressed the proliferation of splenic CD4+ T cells and significantly reduced atherosclerosis in recipient mice. Conclusions Our study has identified an athero-protective role for CD8+CD25+ T cells in experimental atherosclerosis. |
| Databáze: | OpenAIRE |
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