Chromate resistance plasmid in Pseudomonas fluorescens
| Autor: | Ananda M. Chakrabarty, L. H. Bopp, H. L. Ehrlich |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Plasmid preparation
biology R Factors Mitomycin C Pseudomonas fluorescens biology.organism_classification medicine.disease_cause Microbiology Anti-Bacterial Agents Transformation (genetics) Plasmid Plasmid dna Genes Bacterial Metals Mutation medicine Chromates DNA Transposable Elements CHROMATE RESISTANCE Molecular Biology Escherichia coli Research Article |
| Zdroj: | Journal of bacteriology. 155(3) |
| ISSN: | 0021-9193 |
| Popis: | Chromate resistance of Pseudomonas fluorescens LB300, isolated from chromium-contaminated sediment in the upper Hudson River, was found to be plasmid specified. Loss of the plasmid (pLHB1) by spontaneous segregation or mitomycin C curing resulted in a simultaneous loss of chromate resistance. Subsequent transformation of such strains with purified pLHB1 plasmid DNA resulted in a simultaneous re-acquisition of the chromate resistance phenotype and the plasmid. When pLHB1 was transferred by conjugation to Escherichia coli, the plasmid still conferred chromate resistance. |
| Databáze: | OpenAIRE |
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