Relationship Between Plasma Osteopontin and Arginine Pathway Metabolites in Patients With Overt Coronary Artery Disease
| Autor: | Donato Moschetta, Matteo Nicola Dario Di Minno, Benedetta Porro, Gianluca L. Perrucci, Vincenza Valerio, Valentina Alfieri, Ilaria Massaiu, Alexander N. Orekhov, Alessandro Di Minno, Paola Songia, Viviana Cavalca, Veronika A. Myasoedova, Paolo Poggio |
|---|---|
| Přispěvatelé: | Moschetta, D., Di Minno, M. N. D., Porro, B., Perrucci, G. L., Valerio, V., Alfieri, V., Massaiu, I., Orekhov, A. N., Di Minno, A., Songia, P., Cavalca, V., Myasoedova, V. A., Poggio, P. |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
OPN medicine.medical_specialty Arginine Physiology 030204 cardiovascular system & hematology medicine.disease_cause lcsh:Physiology endothelial dysfunction 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine atherosclerosi stomatognathic system Enos nitric oxide Physiology (medical) Internal medicine medicine Citrulline Osteopontin Endothelial dysfunction lcsh:QP1-981 biology business.industry Ornithine Brief Research Report medicine.disease biology.organism_classification Arginase 030104 developmental biology Endocrinology chemistry citrulline biology.protein atherosclerosis business Oxidative stress |
| Zdroj: | Frontiers in Physiology Frontiers in Physiology, Vol 11 (2020) |
| ISSN: | 1664-042X |
| Popis: | Introduction Osteopontin (OPN) is involved in ectopic calcification. Its circulating form is upregulated in coronary artery disease (CAD) patients. Circulating OPN levels positively correlate with oxidative stress, one of the major triggers of endothelial dysfunction. Endothelial dysfunction is, in turn, associated with reduced nitric oxide (NO) bioavailability due to the impaired arginine pathway. The aim of this study was to better understand the correlations between OPN, oxidative stress markers, and the arginine pathway metabolites. Methods and results ELISA and mass spectrometry techniques have been used to evaluate circulating OPN and arginine pathway/oxidative stress metabolites, respectively, in twenty-five control subjects and thirty-three patients with overt atherosclerosis. OPN positively correlates with 2,3-dinor-8isoPGF2a levels (p = 0.02), ornithine (p = 0.01), ADMA (p = 0.001), SDMA (p = 0.03), and citrulline (p = 0.008) levels only in CAD patients. In addition, citrulline positively correlated with ADMA (p = 0.02) levels, possibly as result of other sources of citrulline biosynthetic pathways. Conclusion The association between OPN and impaired arginine/NO pathway could play a role in the inhibition of endothelial NO synthase (eNOS) and/or in the arginase activation in the context of CAD patients. However, further studies are needed to verify the cause-effect relationship between OPN, oxidative stress, and arginine/NO pathway dysregulation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|