Fluctuations in Blood Marginal Zone B-Cell Frequencies May Reflect Migratory Patterns Associated with HIV-1 Disease Progression Status

Autor: Slow Progressor Cohorts, Johanne Poudrier, Michel Roger, Montreal Primary Hiv Infection, Josiane Chagnon-Choquet, Julie Gauvin
Rok vydání: 2016
Předmět:
RNA viruses
0301 basic medicine
Chemokine
B Cells
Physiology
lcsh:Medicine
HIV Infections
Pathology and Laboratory Medicine
White Blood Cells
Immunodeficiency Viruses
Animal Cells
B-Cell Activating Factor
Blood plasma
Marginal zone B-cell
Medicine and Health Sciences
Public and Occupational Health
lcsh:Science
B-Lymphocytes
education.field_of_study
Multidisciplinary
Chemotaxis
Cell migration
Hematology
Marginal zone
Vaccination and Immunization
Body Fluids
Cell Motility
Blood
Infectious Diseases
Medical Microbiology
Chemokines
CC
Viral Pathogens
Viruses
Disease Progression
Cellular Types
Anatomy
Chemokines
Pathogens
Research Article
Adult
Receptors
CXCR4
Infectious Disease Control
Immune Cells
Immunology
Population
Antiretroviral Therapy
Biology
Microbiology
Blood Plasma
Young Adult
03 medical and health sciences
Immune system
Antiviral Therapy
Retroviruses
Humans
Antibody-Producing Cells
B-cell activating factor
education
Microbial Pathogens
Blood Cells
lcsh:R
Lentivirus
Organisms
Biology and Life Sciences
HIV
Cell Biology
Memory B cells
Chemokine CXCL13
Chemokine CXCL12
030104 developmental biology
Socioeconomic Factors
HIV-1
biology.protein
lcsh:Q
Preventive Medicine
Zdroj: PLoS ONE
PLoS ONE, Vol 11, Iss 5, p e0155868 (2016)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0155868
Popis: We have previously shown that overexpression of BLyS/BAFF was associated with increased relative frequencies of innate "precursor" marginal zone (MZ)-like B-cells in the blood of HIV-1-infected rapid and classic progressors. However, along with relatively normal BLyS/BAFF expression levels, these cells remain unaltered in elite-controllers (EC), rather, percentages of more mature MZ-like B-cells are decreased in the blood of these individuals. Fluctuations in frequencies of blood MZ-like B-cell populations may reflect migratory patterns associated with disease progression status, suggesting an important role for these cells in HIV-1 pathogenesis. We have therefore longitudinally measured plasma levels of B-tropic chemokines by ELISA-based technology as well as their ligands by flow-cytometry on blood B-cell populations of HIV-1-infected individuals with different rates of disease progression and uninfected controls. Migration potential of B-cell populations from these individuals were determined by chemotaxis assays. We found important modulations of CXCL13-CXCR5, CXCL12-CXCR4/CXCR7, CCL20-CCR6 and CCL25-CCR9 chemokine-axes and increased cell migration patterns in HIV progressors. Interestingly, frequencies of CCR6 expressing cells were significantly elevated within the precursor MZ-like population, consistent with increased migration in response to CCL20. Although we found little modulation of chemokine-axes in EC, cell migration was greater than that observed for uninfected controls, especially for MZ-like B-cells. Overall the immune response against HIV-1 may involve recruitment of MZ-like B-cells to peripheral sites. Moreover, our findings suggest that "regulated" attraction of these cells in a preserved BLyS/BAFF non-inflammatory environment, such as encountered in EC could be beneficial to the battle and even control of HIV.
Databáze: OpenAIRE
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