Galectin-9-like from Angiostrongylus cantonensis young adult worms modulates eosinophil chemotaxis in vitro
| Autor: | Chuan-Min Yen, Jiun-Jye Wang, Zong-Sheng Wu, Chi-Yu Lu, Li-Yu Chung |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Microbiology (medical) Galectins Blotting Western 030106 microbiology lcsh:QR1-502 Biology Mass Spectrometry lcsh:Microbiology Eosinophil chemotactic activity Mice 03 medical and health sciences 0302 clinical medicine Western blot medicine Animals Immunology and Allergy 030212 general & internal medicine Galectin Life Cycle Stages Mice Inbred BALB C General Immunology and Microbiology medicine.diagnostic_test Galectin-9-like Chemotaxis Brain Angiostrongylus cantonensis General Medicine Eosinophil biology.organism_classification Molecular biology In vitro Eosinophils Infectious Diseases medicine.anatomical_structure Cerebrospinal fluid Eosinophil chemotaxis Female Angiostrongylus |
| Zdroj: | Journal of Microbiology, Immunology and Infection, Vol 53, Iss 4, Pp 604-611 (2020) |
| ISSN: | 1684-1182 |
| Popis: | Background/purpose Eosinophils are recruited to the brain of mice after infection with Angiostrongylus canonensis. Several factors produced by infected mice are well known playing the role to chemoattract eosinophils from the blood into the brain. The purpose of this study is to investigate whether Angiostronylus cantonensis young-adult worms (AcYA) have components which have eosinophilic chemotactic activity. Methods Eosinophil chemotactic activity of AcYA was tested by Boyden blind-well chamber technique. The components of AcYA were analysed by SDS-PAGE and Mass spectrometry. Furthermore, galectin-9 in the cerebrospinal fluid (CSF) of infected mice and galectin-9-like in AcYA were measured by ELISA technic and also were recognized by western blot analysis respectively. Results Excretory-secretory products of AcYA did not show eosinophil chemotactic activity. However, the extracts of AcYA showed protein concentration-dependent eosinophil chemotactic activity and reached the peak at the 24 μg/ml. The eosinophil chemotactic activity was significantly reduced by lactose. The components of AcYA at molecular weights of approximatively 15 kDa and 35 kDa showed several galectins component in Mass spectrometric analysis. Furthermore, galectin-9-like in AcYA was recognized by ELISA and western blot analysis. In parallel with increase of galectin-9 in the CSF, eosinophils were also significantly increased in mouse after infected with A. cantonensis. Conclusion Galectin-9-like in AcYA and galectin-9 in mouse CSF were confirmed demonstrating eosinophil chemotactic activity both in vitro study and in the infection of mouse in this study. |
| Databáze: | OpenAIRE |
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