Tracking superparamagnetic iron oxide labeled monocytes in brain by high-field magnetic resonance imaging
| Autor: | Jay A. Nelson, Melissa Mellon, Mariano G. Uberti, Larissa Poluektova, Marina Zelivyanskaya, Howard E. Gendelman, Michael D. Boska |
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| Rok vydání: | 2003 |
| Předmět: |
Nervous system
Male Pathology medicine.medical_specialty Cell Survival Iron Neuropathology Mice SCID Corpus callosum Monocytes Cellular and Molecular Neuroscience Mice In vivo Cell Movement medicine Animals Humans Magnetite Nanoparticles Cells Cultured medicine.diagnostic_test Microglia business.industry Phantoms Imaging Monocyte Brain Magnetic resonance imaging Cell migration Dextrans Oxides Immunohistochemistry Magnetic Resonance Imaging Ferrosoferric Oxide medicine.anatomical_structure Blood-Brain Barrier business Neuroscience |
| Zdroj: | Journal of neuroscience research. 73(3) |
| ISSN: | 0360-4012 |
| Popis: | Inflammatory cells, most notably mononuclear phagocytes (MP; macrophages and microglia), play a critical role in brain homeostasis, repair and disease. One important event in cellular biodynamics is how MP move in and throughout the nervous system. Prior studies have focused principally on cell migration across the blood-brain barrier during neuroinflammatory processes with little work done on cell movement within the brain. During the past decade our laboratories have studied the role of MP in HIV-1-associated dementia (HAD). In HAD MP incite sustained glial inflammatory reactions causing significant neuronal damage. To extend these works we investigated cell movement in brain and its influence for disease in a novel co-registration system integrating neuropathology with high-field magnetic resonance imaging (MRI). Human monocytes labeled with superparamagnetic iron oxide particles were injected into the brain of severe combined immunodeficient (SCID) mice. MRI was recorded 1, 7, and 14 days after cell injection. MRI co-registered with histology verified that the MRI signal modification was due to the labeled cells. MRI showed human monocyte-derived macrophages along the injection site, the corpus callosum, the ventricular system and in other brain sites. These data support the idea that cell migration can be monitored in vivo and provides an opportunity to assess monocyte mobility in brain and its affects on neurodegenerative processes and notably HAD. |
| Databáze: | OpenAIRE |
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