Identification of ligands for two human bitter T2R receptors
| Autor: | Judy Connor, Walter Keung, Alexey Pronin, Huixian Tang |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Physiology
G protein Molecular Sequence Data Gene Expression Biology Spodoptera Ligands Receptors G-Protein-Coupled Behavioral Neuroscience chemistry.chemical_compound Saccharin stomatognathic system Physiology (medical) Extracellular Animals Humans Amino Acid Sequence Receptor Cells Cultured Phylogeny Binding Sites Functional analysis Ligand Denatonium Sensory Systems Recombinant Proteins Quaternary Ammonium Compounds Biochemistry chemistry Multigene Family Taste Human genome Signal transduction Sequence Alignment psychological phenomena and processes |
| Zdroj: | Chemical senses. 29(7) |
| ISSN: | 0379-864X |
| Popis: | Earlier, a family of G protein-coupled receptors, termed T2Rs, was identified in the rodent and human genomes through data mining. It was suggested that these receptors mediate bitter taste perception. Analysis of the human genome revealed that the hT2R family is composed of 25 members. However, bitter ligands have been identified for only three human receptors so far. Here we report identification of two novel ligand–receptor pairs. hT2R61 is activated by 6-nitrosaccharin, a bitter derivative of saccharin. hT2R44 is activated by denatonium and 6-nitrosaccharin. Activation profiles for these receptors correlate with psychophysical data determined for the bitter compounds in human studies. Functional analysis of hT2R chimeras allowed us to identify residues in extracellular loops critical for receptor activation by ligands. The discovery of two novel bitter ligand– receptor pairs provides additional support for the hypothesis that hT2Rs mediate a bitter taste response in humans. |
| Databáze: | OpenAIRE |
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