Expression and Secretion of Cholera Toxin B Subunit in Lactobacilli
| Autor: | Yukihiko Nakashima, Tanjina Sharmin, Seitaro Yamada, Keiichi Irie, Takeshi Miyata, Tomomitsu Satho, Saori Uyeda, Fumio Miake, Tetsuya Harakuni, Masumi Imoto, Nobuhiro Kashige, Takeshi Arakawa, Yukihiro Hiramatsu, Akihisa Toda, Yuki Fukumitsu, Takahiro Okuno |
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| Rok vydání: | 2013 |
| Předmět: |
Cholera Toxin
Pharmaceutical Science medicine.disease_cause complex mixtures Microbiology Mice Antigen Gangliosides Generally recognized as safe Escherichia coli medicine Animals Pharmacology Antigens Bacterial Mice Inbred BALB C biology Immunogenicity Cholera toxin Cholera Vaccines General Medicine biology.organism_classification medicine.disease Antibodies Bacterial Cholera Recombinant Proteins Lactobacillus Vibrio cholerae Immunoglobulin G embryonic structures Female Cholera vaccine Bacteria Plasmids |
| Zdroj: | Biological and Pharmaceutical Bulletin. 36:952-958 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.b12-01021 |
| Popis: | Lactic acid bacteria (LAB) are used in various fields, including in food and medical supplies. There has been a great deal of research into vaccine development using LAB as carriers due to their "generally recognized as safe" status. Cholera is an infectious disease that causes diarrhea due to cholera toxin (CT) produced by Vibrio cholerae. The pentameric cholera toxin B (CTB) subunit has no toxicity, and is used as an antigen in cholera vaccines and as a delivery molecule in vaccines to various diseases. In this study, we generated recombinant LAB expressing and secreting CTB. Here, we first report that CTB expressed and secreted from LAB bound to GM1 ganglioside. The secreted CTB was purified, and its immunogenicity was determined by intranasal administration into mice. The results of the present study suggested that it may be useful as the basis of a new oral cholera vaccine combining LAB and CTB. |
| Databáze: | OpenAIRE |
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