In Vitro Protective Effects of Two Extracts from Bergamot Peels on Human Endothelial Cells Exposed to Tumor Necrosis Factor-α (TNF-α)
| Autor: | Antonella Saija, Domenico Trombetta, Francesco Cimino, Keith W. Waldron, Mariateresa Cristani, Arjan Narbad, Antonio Speciale, Giuseppina Mandalari, Giovanna Ginestra, Giuseppe Bisignano, Joselita Chirafisi, Craig B. Faulds |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Citrus
antioxidant Antioxidant Endothelium TNF-alfa medicine.medical_treatment Protective Agents medicine.disease_cause Cell Line SOS chromotest Superoxide dismutase medicine human vein endothelial cells Humans Bergamot peels biology Plant Extracts Tumor Necrosis Factor-alpha Endothelial Cells food and beverages General Chemistry Endothelial stem cell Cytokine medicine.anatomical_structure Biochemistry Fruit Citrus bergamia biology.protein Tumor necrosis factor alpha General Agricultural and Biological Sciences Genotoxicity |
| Zdroj: | Journal of Agricultural and Food Chemistry. 58:8430-8436 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf1008605 |
| Popis: | Bergamot ( Citrus bergamia Risso) is a less commercialized Citrus fruit, mainly used for its essential oil extracted from the peel. Bergamot peel (BP) represents about 60% of the processed fruits and is regarded as primary waste. However, it contains good amounts of useful compounds, such as pectins and flavonoids. Many of the bioactivities of Citrus flavonoids appear to impact vascular endothelial cells. Herein, we report the protective effect of two flavonoid-rich extracts from BP (endowed with radical-scavenging properties and lacking genotoxic activity) against alterations in cell modifications induced by the pleiotropic inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) on human umbilical vein endothelial cells (HUVECs), as demonstrated by monitoring intracellular levels of malondialdehyde/4-hydroxynonenal, reduced and oxidized glutathione and superoxide dismutase activity, and the activation status of nuclear factor-kappaB (NF-kappaB). Thus, BP appears to be a potential source of natural antioxidant/anti-inflammatory phytocomplexes to be employed as ingredients of nutraceutical products or functional foods. |
| Databáze: | OpenAIRE |
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