Characterization of the protein processing and secretion pathways in a comprehensive set of expressed sequence tags from Trichoderma reesei
| Autor: | Thomas D. Houfek, Thomas K. Mitchell, Stephen E. Diener, P. van Solingen, Pauline Johanna Maria Teunissen, Michael South San Francisco Ward, Lydia Dankmeyer, Pamela K. Foreman, Nigel Dunn-Coleman, Ralph A. Dean |
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| Rok vydání: | 2004 |
| Předmět: |
Genetics
Expressed Sequence Tags Trichoderma Expressed sequence tag biology Aspergillus niger Computational Biology Sequence Analysis DNA biology.organism_classification Microbiology Sequence-tagged site Fungal Proteins Protein Transport Complementary DNA Protein folding Molecular Biology Gene Protein Processing Post-Translational Secretory pathway Trichoderma reesei Gene Library |
| Zdroj: | FEMS microbiology letters. 230(2) |
| ISSN: | 0378-1097 |
| Popis: | Trichoderma reesei is a filamentous fungus widely used as an efficient protein producer and known to secrete large quantities of biomass degrading enzymes. Much work has been done aimed at improving the secretion efficiency of this fungus. It is generally accepted that the major bottlenecks in secretion are protein folding and ornamentation steps in this pathway. In an attempt to identify genes involved in these steps, the 5′ ends of 21 888 cDNA clones were sequenced from which a unique set of over 5000 were also 3′ sequenced. Using annotation tools Gene Ontology terms were assigned to 2732 of the sequences. Homologs to the majority of Aspergillus niger ’s Srg genes as well as a number of homologs to genes involved in protein folding and ornamentation pathways were identified. |
| Databáze: | OpenAIRE |
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