Thyroxine replacement modifies changes in deiodinase and thyroid hormone transporter expression induced by subclinical hypothyroidism in rats
| Autor: | Kelen Carneiro Oliveira, Roberto Laureano-Melo, Rodrigo Rodrigues da Conceição, Gisele Giannocco, Rui M. B. Maciel, Janaina Sena de Souza, Monica Akemi Sato, Maria Izabel Chiamolera, Wellington da Silva Côrtes |
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| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism CRYM Deiodinase Hypothalamus DIO2 030209 endocrinology & metabolism 030204 cardiovascular system & hematology Iodide Peroxidase 03 medical and health sciences Thyroxine-Binding Proteins 0302 clinical medicine Hypothyroidism Internal medicine mu-Crystallins Gene expression Medicine Animals heterocyclic compounds RNA Messenger Kidney biology business.industry Thyroid General Medicine Rats Thyroxine Endocrinology medicine.anatomical_structure nervous system Gene Expression Regulation biology.protein business Hormone |
| Zdroj: | Hormones (Athens, Greece). 20(1) |
| ISSN: | 2520-8721 |
| Popis: | The potential benefits of treating subclinical hypothyroidism (SCH) are unclear and still controversial. Thus, we surgically induced SCH in rats and evaluated the effects of thyroxine (T4) replacement on the gene expression levels of deiodinases and thyroid hormone (TH) transporters in different tissues. SCH was induced by hemithyroid electrocauterization. The control animals underwent the same surgical procedure but were not subjected to electrocauterization (sham). After 14 days, half of the SCH animals were treated with T4 (SCH + T4). At the end of the experimental protocol, all of the rats were euthanized, serum hormone concentrations were measured, and RNA analyses were performed on different tissues and organs. Consistent with previous studies, we observed increased TSH levels, normal TH levels, and reduced hypothalamic TRH expression in the SCH group. Additionally, Dio2 mRNA expression was downregulated in the hippocampus and pituitary, and Dio1 was upregulated in the kidney and pituitary of the SCH animals. The changes in Dio3 expression were tissue-specific. Concerning TH transporters, Mct10 expression was upregulated in the pituitary, kidney, hypothalamus, and hippocampus, and Mct8 expression was downregulated in the kidney of the SCH group. Crym expression was upregulated in the kidney and pituitary. Notably, T4 replacement significantly attenuated serum TSH levels and reverted Dio1, Dio2, Mct10, and Crym expression in the pituitary, hippocampus, and kidney to levels that were similar to the sham group. Tissue-specific responses were also observed in the liver and hypothalamus. Our results indicate that treatment of SCH should be considered before the appearance of clinical symptoms of hypothyroidism. |
| Databáze: | OpenAIRE |
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