A Role for Arabidopsis miR399f in Salt, Drought, and ABA Signaling

Autor: Dongwon Baek, Doh Hoon Kim, Min Chul Kim, S.G. Kang, Gilok Shin, Su Jung Park, Hyewon Hong, Sang Yeol Lee, Chanmin Kim, Hyun Jin Chun, Dae-Jin Yun
Rok vydání: 2015
Předmět:
0106 biological sciences
0301 basic medicine
Salinity
Arabidopsis
drought
Sodium Chloride
01 natural sciences
chemistry.chemical_compound
Gene Expression Regulation
Plant
Genes
Reporter
Gene expression
Arabidopsis thaliana
salt
Abscisic acid
biology
microRNA
beta-Glucosidase
food and beverages
General Medicine
Plants
Genetically Modified
Adaptation
Physiological
Cell biology
Droughts
Basic-Leucine Zipper Transcription Factors
ABA
Signal Transduction
abiotic stress
Transgene
Recombinant Fusion Proteins
Article
Phosphates
03 medical and health sciences
Stress
Physiological
Botany
Molecular Biology
Gene
Abiotic stress
Arabidopsis Proteins
fungi
Globulins
Cell Biology
biology.organism_classification
Phosphate
MicroRNAs
030104 developmental biology
chemistry
Trans-Activators
010606 plant biology & botany
Abscisic Acid
Zdroj: Molecules and Cells
ISSN: 0219-1032
Popis: MiR399f plays a crucial role in maintaining phosphate homeostasis in Arabidopsis thaliana. Under phosphate starvation conditions, AtMYB2, which plays a role in plant salt and drought stress responses, directly regulates the expression of miR399f. In this study, we found that miR399f also participates in plant responses to abscisic acid (ABA), and to abiotic stresses including salt and drought. Salt and ABA treatment induced the expression of miR399f, as confirmed by histochemical analysis of promoter-GUS fusions. Transgenic Arabidopsis plants overexpressing miR399f (miR399f-OE) exhibited enhanced tolerance to salt stress and exogenous ABA, but hypersensitivity to drought. Our in silico analysis identified ABF3 and CSP41b as putative target genes of miR399f, and expression analysis revealed that mRNA levels of ABF3 and CSP41b decreased remarkably in miR399f-OE plants under salt stress and in response to treatment with ABA. Moreover, we showed that activation of stress-responsive gene expression in response to salt stress and ABA treatment was impaired in miR399f-OE plants. Thus, these results suggested that in addition to phosphate starvation signaling, miR399f might also modulates plant responses to salt, ABA, and drought, by regulating the expression of newly discovered target genes such as ABF3 and CSP41b.
Databáze: OpenAIRE
Externí odkaz: