Quantification of ketoprofen enantiomers in human plasma based on solid-phase extraction and enantioselective column chromatography
| Autor: | Saeed A Qureshi, Iain J. McGilveray, Gilles Caillé, Julie Boisvert |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Ketoprofen
Chromatography Chemistry Coefficient of variation Anti-Inflammatory Agents Non-Steroidal Extraction (chemistry) Reproducibility of Results Stereoisomerism General Chemistry Sensitivity and Specificity High-performance liquid chromatography chemistry.chemical_compound Column chromatography medicine Humans Solid phase extraction Enantiomer Ammonium acetate Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 690:189-193 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(96)00402-1 |
| Popis: | An HPLC method for the quantification of ketoprofen enantiomers in human plasma is described. Following extraction with a disposable C 18 solid-phase extraction column, separation of ketoprofen enantiomers and I.S. (3,4-dimethoxy benzoic acid) was achieved using a chiral column [Chirex 3005; ( R )-1-naphthylglycine 3,5-dinitrobenzoic acid] with the mobile phase, 0.02 M ammonium acetate in methanol, set at a flow-rate of 1.2 ml/min. Baseline separation of ketoprofen enantiomers and I.S., free from interferences, was achieved in less than 20 min. The calibration curves ( n = 14) were linear over the concentration range of 0.16 to 5.00 μg/ml per enantiomer [mean r 2 of 0.999 for both enantiomers, root mean square error were 0.015 for R (−) and 0.013 for S (+)]. The inter-day coefficient of variation for duplicate analysis of spiked samples was less than 7% and the accuracy was more than 93% over the concentration range of 0.2 to 4.0 μg/ml for individual enantiomer using 1 ml of plasma sample. This method has been applied to a pharmacokinetic study from healthy human volunteers following the administration of a ketoprofen extended release product (200 mg). This method is simple, fast and should find wide application in monitoring pharmacokinetic studies of ketoprofen. |
| Databáze: | OpenAIRE |
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