Changes in Cellular Localization of Inter-Alpha Inhibitor Proteins after Cerebral Ischemia in the Near-Term Ovine Fetus

Autor:	Boram Kim, Alistair J. Gunn, Joanne O. Davidson, Yow-Pin Lim, Barbara S. Stonestreet, Laura Bennet, Kazuki Hatayama, Xiaodi Chen
Rok vydání:	2021
Předmět:	Male QH301-705.5 ovine fetus Neuroprotection Article Catalysis Inorganic Chemistry Fetus Alpha-Globulins medicine Animals Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology Spectroscopy Cellular localization Neurons Sheep biology Microglia Chemistry Endoplasmic reticulum Organic Chemistry General Medicine brain injury Subcellular localization Computer Science Applications Cell biology Cortex (botany) Oligodendroglia hypoxia-ischemia Neuroprotective Agents medicine.anatomical_structure Animals Newborn inter-alpha inhibitor proteins nervous system Cerebral cortex Hypoxia-Ischemia Brain biology.protein Female NeuN Subcellular Fractions
Zdroj:	International Journal of Molecular Sciences Volume 22 Issue 19 International Journal of Molecular Sciences, Vol 22, Iss 10751, p 10751 (2021)
ISSN:	1422-0067
DOI:	10.3390/ijms221910751
Popis:	Inter-alpha Inhibitor Proteins (IAIPs) are key immunomodulatory molecules. Endogenous IAIPs are present in human, rodent, and sheep brains, and are variably localized to the cytoplasm and nuclei at multiple developmental stages. We have previously reported that ischemia-reperfusion (I/R) reduces IAIP concentrations in the fetal sheep brain. In this study, we examined the effect of I/R on total, cytoplasmic, and nuclear expression of IAIPs in neurons (NeuN+), microglia (Iba1+), oligodendrocytes (Olig2+) and proliferating cells (Ki67+), and their co-localization with histones and the endoplasmic reticulum in fetal brain cells. At 128 days of gestation, fetal sheep were exposed to Sham (n = 6) or I/R induced by cerebral ischemia for 30 min with reperfusion for 7 days (n = 5). Although I/R did not change the total number of IAIP+ cells in the cerebral cortex or white matter, cells with IAIP+ cytoplasm decreased, whereas cells with IAIP+ nuclei increased in the cortex. I/R reduced total neuronal number but did not change the IAIP+ neuronal number. The proportion of cytoplasmic IAIP+ neurons was reduced, but there was no change in the number of nuclear IAIP+ neurons. I/R increased the number of microglia and decreased the total numbers of IAIP+ microglia and nuclear IAIP+ microglia, but not the number of cytoplasmic IAIP+ microglia. I/R was associated with reduced numbers of oligodendrocytes and increased proliferating cells, without changes in the subcellular IAIP localization. IAIPs co-localized with the endoplasmic reticulum and histones. In conclusion, I/R alters the subcellular localization of IAIPs in cortical neurons and microglia but not in oligodendrocytes or proliferating cells. Taken together with the known neuroprotective effects of exogenous IAIPs, we speculate that endogenous IAIPs may play a role during recovery from I/R.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc97b237bcfc0cb67458e3637688c3e6 https://doi.org/10.3390/ijms221910751 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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