IsFusarium culmorumisotrichodermin-15-hydroxylase different from other fungal species?
| Autor: | Lolita O. Zamir, Carole Abi Farah |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Fusarium
Metabolite Immunology Trichothecene Coenzymes Applied Microbiology and Biotechnology Microbiology Chromatography Affinity Fungal Proteins chemistry.chemical_compound Cytochrome P-450 Enzyme System Microsomes Genetics Fusarium culmorum Mycotoxin Molecular Biology Chromatography High Pressure Liquid chemistry.chemical_classification Cell-Free System Flavoproteins biology food and beverages Cytochrome P450 General Medicine Fungi imperfecti biology.organism_classification Enzyme Biochemistry chemistry biology.protein Electrophoresis Polyacrylamide Gel Trichothecenes |
| Zdroj: | Canadian Journal of Microbiology. 46:143-149 |
| ISSN: | 1480-3275 0008-4166 |
| Popis: | Fusarium spp. are ubiquitous fungi infecting cereals and grains, and therefore constitute a major problem for agriculture. Their trichothecene metabolites, and in particular deoxynivalenol and its 3-acetylated derivative, are the mycotoxins involved. The major metabolite produced by Fusarium culmorum is 3-acetyldeoxynivalenol. Studies in vivo with Fusarium culmorum have established that its tricyclic intermediate, isotrichodermin, is a major biosynthetic precursor, which is hydroxylated at position 15 to give 15-deacetylcalonectrin, prior to being converted to the product. In a preliminary in vitro investigation of the cell-free system involved in this transformation, we suggested that cytochrome P450 enzymes are not involved. In this paper, the isotrichodermin-15-hydroxylase from the microsomal fraction of Fusarium culmorum was solubilized and partially purified (60 fold). Our studies with cofactors indicate that this enzyme is a flavoprotein, and the inducers tested highly indicate that indeed the hydroxylase is not attached to cytochrome P450. This is particularly interesting, since the only other enzyme catalyzing the same reaction isolated from Fusarium sporotrichiodes is attached to cytochrome P450. Key words: trichothecene, Fusarium culmorum, cell-free system, isotrichodermin, 15-deacetylcalonectrin, flavoprotein. |
| Databáze: | OpenAIRE |
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