Detection of canine parvovirus types 2b and 2c in canine faecal samples contaminating urban thoroughfares in Brazil
| Autor: | Izabel Carolina Vargas Pinto Gogone, Amauri Alcindo Alfieri, Flavia Regina Oliveira de Barros, Elisabete Takiuchi, Flávia Possatti |
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| Rok vydání: | 2019 |
| Předmět: |
Mitochondrial DNA
Parvovirus Canine 040301 veterinary sciences Sequence analysis animal diseases viruses Immunology Pcr assay Applied Microbiology and Biotechnology Microbiology DNA Mitochondrial Polymerase Chain Reaction Virus 0403 veterinary science Parvoviridae Infections 03 medical and health sciences Feces Dogs Genetics Environmental Microbiology Animals Dog Diseases Molecular Biology Phylogeny 030304 developmental biology 0303 health sciences Phylogenetic tree biology Canine parvovirus 04 agricultural and veterinary sciences General Medicine Acute gastroenteritis biology.organism_classification Virology Gastroenteritis Brazil |
| Zdroj: | Canadian journal of microbiology. 66(2) |
| ISSN: | 1480-3275 |
| Popis: | Canine parvovirus type 2 (CPV-2) is a highly contagious virus that causes acute gastroenteritis in dogs all over the world. Because of its stability in the environment, CPV-2 can remain infective for a long time, especially if protected in organic matter. To demonstrate CPV-2’s potential as an environmental hazard for nonimmunized susceptible hosts, we investigated 50 faecal samples collected from public areas in a municipality of Paraná state, Brazil. Seven samples tested positive for CPV by a PCR assay targeting the partial VP2 gene, with three strains being confirmed as CPV-2b variant and one as CPV-2c variant by sequence analysis. These findings were supported by phylogenetic analysis, and the species identity of faecal samples source was confirmed by canine mitochondrial DNA amplification and sequencing. Our results demonstrate the presence of CPV in canine faeces contaminating urban thoroughfares and reinforce the importance of environmental control to reduce the potential exposure risks to susceptible hosts. |
| Databáze: | OpenAIRE |
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