Association of CYP26C1 Promoter Hypomethylation with Small Vessel Occlusion in Korean Subjects
| Autor: | Nam-Hui Yim, Eun-Ji Lee, Min Ho Cha, Myung-Sunny Kim |
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| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty blood parameter Hematocrit QH426-470 Article Epigenesis Genetic chemistry.chemical_compound Internal medicine Diabetes mellitus Lactate dehydrogenase Republic of Korea medicine Genetics retinoic acid Humans Epigenetics Vascular Diseases Promoter Regions Genetic Genetics (clinical) Cytochrome P450 Family 26 Aged Binding Sites DNA methylation medicine.diagnostic_test business.industry Promoter Methylation Middle Aged medicine.disease small vessel occlusion Endocrinology CYP26C1 CpG site chemistry Female business Transcription Factors |
| Zdroj: | Genes Volume 12 Issue 10 Genes, Vol 12, Iss 1622, p 1622 (2021) |
| ISSN: | 2073-4425 |
| Popis: | The risk factors for stroke, a fatal disease, include type two diabetes, hypertension, and genetic influences. Small vessel occlusion (SVO) can be affected by epigenetic alterations, but an association between SVO and the methylation of cytochrome P450 family 26 subfamily C member 1 (CYP26C1) has not been identified. In this study, we measured the level of DNA methylation in the CYP26C1 promoter and the 5′ untranslated region of 115 normal subjects and 56 patients with SVO in Korea. The DNA methylation level of each subject was measured by bisulfite amplicon sequencing, and statistical analysis was performed using the general linear model or Pearson’s correlation. The average level of DNA methylation was markedly lower in patients with SVO than in normal subjects (20.4% vs. 17.5%). We found that the methylation of CYP26C1 has a significant positive correlation with blood parameters including white blood cells, hematocrit, lactate dehydrogenase, and Na+ in subjects with SVO. We predicted that binding of RXR-α and RAR-β might be affected by CYP26C1 methylation at CpG sites −246–237 and −294–285. These findings suggest that CYP26C1 methylation in the promoter region may be a predictor of SVO. |
| Databáze: | OpenAIRE |
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