Identification of the gene encoding the 5S ribosomal RNA maturase in Bacillus subtilis: Mature 5S rRNA is dispensable for ribosome function
| Autor: | Beltcho Beltchev, Harald Putzer, Dominique Brechemier-Baey, Ciarán Condon, Marianne Grunberg-Manago |
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| Rok vydání: | 2001 |
| Předmět: |
Cell Survival
RNase P Molecular Sequence Data Biology Polymerase Chain Reaction Ribosome RNase PH 5S ribosomal RNA Endoribonucleases RNA Precursors Amino Acid Sequence RNA Processing Post-Transcriptional Ribonuclease M5 Base Pairing Molecular Biology DNA Primers Cell Nucleus Genetics Base Sequence Sequence Homology Amino Acid RNA Ribosomal 5S RNA Ribosomal RNA Blotting Northern Nucleotidyltransferases RNA Bacterial RNase MRP Electrophoresis Polyacrylamide Gel Ribosomes Bacillus subtilis Research Article |
| Zdroj: | RNA. 7:242-253 |
| ISSN: | 1355-8382 |
| DOI: | 10.1017/s1355838201002163 |
| Popis: | Over 25 years ago, Pace and coworkers described an activity called RNase M5 in Bacillus subtilis cell extracts responsible for 5S ribosomal RNA maturation (Sogin & Pace, Nature, 1974, 252:598-600). Here we show that RNase M5 is encoded by a gene of previously unknown function that is highly conserved among the low G + C gram-positive bacteria. We propose that the gene be named rnmV. The rnmV gene is nonessential. B. subtilis strains lacking RNase M5 do not make mature 5S rRNA, indicating that this process is not necessary for ribosome function. 5S rRNA precursors can, however, be found in both free and translating ribosomes. In contrast to RNase E, which cleaves the Escherichia coli 5S precursor in a single-stranded region, which is then trimmed to yield mature 5S RNA, RNase M5 cleaves the B. subtilis equivalent in a double-stranded region to yield mature 5S rRNA in one step. For the most part, eubacteria contain one or the other system for 5S rRNA production, with an imperfect division along gram-negative and gram-positive lines. A potential correlation between the presence of RNase E or RNase M5 and the single- or double-stranded nature of the predicted cleavage sites is explored. |
| Databáze: | OpenAIRE |
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