Osteogenic differentiation of Wharton's jelly-derived mesenchymal stem cells cultured on WJ-scaffold through conventional signalling mechanism
| Autor: | Ehsan Taghiabadi, Bahareh Beiki, Bahman Zeynali, Mousa Kehtari, Ehsan Seyedjafari |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Scaffold Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Extracellular matrix 03 medical and health sciences Tissue engineering Biomimetic Materials Cell Movement Osteogenesis parasitic diseases Wharton's jelly Humans Wharton Jelly Tissue Scaffolds Chemistry Mechanism (biology) Mesenchymal stem cell technology industry and agriculture Cell Differentiation Mesenchymal Stem Cells General Medicine Cell biology Extracellular Matrix 030104 developmental biology Signalling Signalling pathways Biotechnology Signal Transduction |
| Zdroj: | Artificial cells, nanomedicine, and biotechnology. 46(sup3) |
| ISSN: | 2169-141X |
| Popis: | Wharton's jelly-derived extracellular matrix (WJ-ECM) has attracted researcher's attention for its biomedical applications. Previously, we fabricated a biomimetic spongy scaffold from decellularized WJ-ECM and, in this study, we sought to examine the osteogenic inductive potential of this scaffold and its underlying mechanism. To address this question, mesenchymal stem cells (MSCs) were isolated from WJ using a mechanical method and cultured on the scaffold, under dynamic condition, for over 21 days in the presence or absence of osteogenic medium. The status of signalling pathways involved in the osteogenic differentiation and the expression profile of integrins in the WJ-derived MSCs (WJ-MSCs) were examined. WJ-MSCs displayed differentiation capacities and expressed surface antigens, characteristics of MSCs. Histologically, WJ-MSCs seeded on the scaffold showed a proper cellular attachment, penetration and migration. They also exhibited a higher degree of alkaline phosphatase activity, calcium deposition and osteogenic gene expression, than those cultured in 2D condition. The expression of Wnt, BMP and TGF-β signalling target genes together with that of α2, αv and β1 integrins was increased in WJ-MSCs in both presence and absence of osteogenic induction medium. Taken together, our results demonstrate that WJ-derived scaffold induces osteogenic differentiation of WJ-MSCs, possibly through activating integrins and subsequently conventional intracellular signalling pathways. |
| Databáze: | OpenAIRE |
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