Design and Characterisation of a Randomized Food Intervention That Mimics Exposure to a Typical UK Diet to Provide Urine Samples for Identification and Validation of Metabolite Biomarkers of Food Intake

Autor: Isabel Garcia-Perez, Kathleen Tailliart, Edward S. Chambers, John Draper, Amanda J. Lloyd, Naomi D Willis, John C. Mathers, Martina Stiegler, Long Xie, Manfred Beckmann
Přispěvatelé: National Institute for Health Research
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Endocrinology
Diabetes and Metabolism
030209 endocrinology & metabolism
lcsh:TX341-641
Urine
CLASSIFICATION
Food group
03 medical and health sciences
POLYPHENOLS
0302 clinical medicine
Environmental health
Medicine
Generalizability theory
STRATEGY
Biomarker discovery
free-living participants
radomized control trial
Nutrition
Original Research
Meal
Science & Technology
030109 nutrition & dietetics
Nutrition and Dietetics
Nutrition & Dietetics
business.industry
GLUCURONIDE
biomarkers
CONSUMPTION
medicine.disease
Obesity
Biobank
metabolomics
POTENTIAL BIOMARKERS
PRODUCTS
INDIVIDUALS
DISCOVERY
Biomarker (medicine)
1111 Nutrition and Dietetics
business
dietary intake
Life Sciences & Biomedicine
lcsh:Nutrition. Foods and food supply
1001 Agricultural Biotechnology
Food Science
Zdroj: Frontiers in Nutrition, Vol 7 (2020)
Frontiers in Nutrition
DOI: 10.3389/fnut.2020.561010/full
Popis: Poor dietary choices are major risk factors for obesity and non-communicable diseases, which places an increasing burden on healthcare systems worldwide. To monitor the effectiveness of healthy eating guidelines and strategies, there is a need for objective measures of dietary intake in community settings. Metabolites derived from specific foods present in urine samples can provide objective biomarkers of food intake (BFIs). Whilst the majority of biomarker discovery/validation studies have investigated potential biomarkers for single foods only, this study considered the whole diet by using menus that delivered a wide range of foods in meals that emulated conventional UK eating patterns. Fifty-one healthy participants (range 19–77 years; 57% female) followed a uniquely designed, randomized controlled dietary intervention, and provided spot urine samples suitable for discovery of BFIs within a real-world context. Free-living participants prepared and consumed all foods and drinks in their own homes and were asked to follow the protocols for meal consumption and home urine sample collection. This study also assessed the robustness, and impact on data quality, of a minimally invasive urine collection protocol. Overall the study design was well-accepted by participants and concluded successfully without any drop outs. Compliance for urine collection, adherence to menu plans, and observance of recommended meal timings, was shown to be very high. Metabolome analysis using mass spectrometry coupled with data mining demonstrated that the study protocol was well-suited for BFI discovery and validation. Novel, putative biomarkers for an extended range of foods were identified including legumes, curry, strongly-heated products, and artificially sweetened, low calorie beverages. In conclusion, aspects of this study design would help to overcome several current challenges in the development of BFI technology. One specific attribute was the examination of BFI generalizability across related food groups and across different preparations and cooking methods of foods. Furthermore, the collection of urine samples at multiple time points helped to determine which spot sample was optimal for identification and validation of BFIs in free-living individuals. A further valuable design feature centered on the comprehensiveness of the menu design which allowed the testing of biomarker specificity within a biobank of urine samples.
Databáze: OpenAIRE
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