Hispidin rescues palmitate-induced insulin resistance in C2C12 myotubes
| Autor: | Eock Kee Hong, Hee Sun Yang, Jun Myoung Park, Hye Jin Lee, Byung Hyun Choi, Jong-Seok Lee, Joo-Hong Yeo, Ki Rim Lee, Sarah Lee |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research medicine.medical_specialty Glucose uptake Muscle Fibers Skeletal Palmitates Biochemistry Cell Line Mice 03 medical and health sciences chemistry.chemical_compound Insulin resistance Internal medicine Genetics medicine Animals Insulin Myocyte Phosphorylation Molecular Biology Protein kinase B biology Myogenesis Adenylate Kinase Skeletal muscle Lipid Metabolism medicine.disease Insulin receptor 030104 developmental biology medicine.anatomical_structure Endocrinology Oncology chemistry Pyrones Cancer research biology.protein Hispidin Molecular Medicine Insulin Resistance Signal Transduction |
| Zdroj: | Molecular Medicine Reports. 16:4229-4234 |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2017.7042 |
| Popis: | Skeletal muscle serves an important role in the utilization of glucose during insulin‑stimulated conditions. Excessive saturated fatty acids are considered to be a major contributing factor to insulin resistance in skeletal muscle cells. The present study investigated the effects of hispidin on palmitate‑induced insulin resistance in C2C12 skeletal muscle myotubes via an MTT assay, glucose uptake assay, Oil‑Red‑O staining and western blot analysis. Hispidin reversed the palmitate‑induced inhibition of glucose uptake, and inhibited palmitate‑induced intracellular lipid accumulation. Hispidin suppressed insulin receptor substrate‑1 Ser307 phosphorylation, and significantly promoted the activation of phosphatidylinositol‑3‑kinase and Akt, via inhibition of protein kinase C theta. Furthermore, hispidin treatment of C2C12 muscle cells increased glucose uptake via activation of adenosine monophosphate‑activated protein kinase. These findings indicated that hispidin may improve palmitate‑induced insulin resistance in skeletal muscle myotubes, and therefore hispidin treatment may be beneficial for patients with diabetes. |
| Databáze: | OpenAIRE |
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