Effects of pex1 disruption on wood lignin biodegradation, fruiting development and the utilization of carbon sources in the white-rot Agaricomycete Pleurotus ostreatus and non-wood decaying Coprinopsis cinerea
| Autor: | Ayako Izuno, Keiji Takabe, Yuji Isagi, Rina Kodera, Kenji Watanabe, Masato Horii, Yoichi Honda, Takashi Watanabe, Hajime Muraguchi, Hiroshi Nishimura, Tatsuya Awano, Yuta Tsunematsu, Yuichiro Hirayama, Takehito Nakazawa, Yasumasa Miyazaki, Masahiro Sakamoto |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Hyphal growth 030106 microbiology Genes Fungal Pleurotus Microbiology Lignin Agaricomycetes Coprinus Fungal Proteins 03 medical and health sciences chemistry.chemical_compound Botany Genetics Peroxisomes Agaricales Biotransformation Mushroom biology fungi food and beverages Peroxisome biology.organism_classification Biological Evolution Carbon Coprinopsis cinerea 030104 developmental biology chemistry Mutagenesis ATPases Associated with Diverse Cellular Activities Pleurotus ostreatus |
| Zdroj: | Fungal genetics and biology : FGB. 109 |
| ISSN: | 1096-0937 |
| Popis: | Peroxisomes are well-known organelles that are present in most eukaryotic organisms. Mutant phenotypes caused by the malfunction of peroxisomes have been shown in many fungi. However, these have never been investigated in Agaricomycetes, which include white-rot fungi that degrade wood lignin in nature almost exclusively and play an important role in the global carbon cycle. Based on the results of a forward genetics study to identify mutations causing defects in the ligninolytic activity of the white-rot Agaricomycete Pleurotus ostreatus, we report phenotypes of pex1 disruptants in P. ostreatus, which are defective in two major features of white-rot Agaricomycetes: lignin biodegradation and mushroom formation. Pex1 disruption was also shown to cause defects in the hyphal growth of P. ostreatus on certain sawdust and minimum media. We also demonstrated that pex1 is essential for fruiting initiation in the non-wood decaying Agaricomycete Coprinopsis cinerea. However, unlike P. ostreatus, significant defects in hyphal growth on the aforementioned agar medium were not observed in C. cinerea. This result, together with previous C. cinerea genetic studies, suggests that the regulation mechanisms for the utilization of carbon sources are altered during the evolution of Agaricomycetes or Agaricales. |
| Databáze: | OpenAIRE |
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