Genotyping and Antifungal Susceptibility Profile of Dipodascus capitatus Isolates Causing Disseminated Infection in Seven Hematological Patients of a Tertiary Hospital
| Autor: | Jose Francisco Tomas, Juan L. Rodriguez-Tudela, Manuel Cuenca-Estrella, Teresa M. Diaz-Guerra, Ignacio Gadea, Jose I. Garcia-Cia, Elena Prieto, Emilia Mellado |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Male Microbiology (medical) Antifungal Agents Genotype Itraconazole Case Report Microbial Sensitivity Tests Biology Polymerase Chain Reaction Flucytosine Microbiology Dipodascus Amphotericin B medicine Humans Typing Mycological Typing Techniques Fungemia Aged Cerebrospinal Fluid Voriconazole Leukemia Middle Aged biology.organism_classification medicine.disease DNA Fingerprinting Hospitals Random Amplified Polymorphic DNA Technique Mycoses Saccharomycetales Female Fluconazole medicine.drug |
| Zdroj: | Journal of Clinical Microbiology. 42:1832-1836 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Seven cases of disseminated infection due to Dipodascus capitatus are reported. Infections occurred in a hematological unit of a tertiary hospital during a period of 5 years. Five cases were refractory to antifungal therapy. Antifungal susceptibility testing of seven isolates was performed, and strains were typed by PCR fingerprinting with the core sequence of phage M13 and by random amplification of polymorphic DNA with two primers, Ap12h and W-80A. A very short range of MICs of each antifungal agent was observed. The MICs of amphotericin B ranged between 0.50 and 2 μg/ml. Strains were susceptible in vitro to flucytosine and susceptible (dose-dependent) to fluconazole and itraconazole. Voriconazole exhibited an activity in vitro comparable to that of itraconazole. Typing techniques allowed seven additional isolates of D. capitatus neither geographically nor temporally related to be classified into two different genomic patterns. The genomic type of the seven strains from the hematological unit was identical regardless of typing technique utilized. It would indicate that the seven cases of disseminated infection could be related epidemiologically. |
| Databáze: | OpenAIRE |
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