Optimization of the Cutoff Value for a Commercial Anti-Dengue Virus IgG Immunoassay
| Autor: | Carolina Sanchez-Vegas, Elizabeth Hunsperger, Luis M. Santiago, Elizabeth D. Barnett, Karla M. Marrero-Santos, Manuela Beltran, Davidson H. Hamer, Jessica Carrión-Lebrón |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Microbiology (medical) medicine.medical_specialty Adolescent viruses Clinical Biochemistry Immunology Enzyme-Linked Immunosorbent Assay Viral Plaque Assay Dengue virus Antibodies Viral medicine.disease_cause Sensitivity and Specificity Immunoglobulin G Dengue fever Serology Dengue Young Adult Plaque reduction neutralization test Neutralization Tests Diagnostic Laboratory Immunology medicine Humans Immunology and Allergy Travel medicine Serologic Tests Child Aged Aged 80 and over Travel biology medicine.diagnostic_test business.industry Yellow fever virus diseases Middle Aged biochemical phenomena metabolism and nutrition medicine.disease Virology ROC Curve Immunoassay biology.protein Female business Boston |
| Zdroj: | Clinical and Vaccine Immunology. 20:358-362 |
| ISSN: | 1556-679X 1556-6811 |
| Popis: | A commercial anti-dengue virus (anti-DENV) indirect IgG enzyme-linked immunosorbent assay (ELISA) for serological diagnosis was evaluated for its utility in determining previous DENV exposure in U.S. travelers. The Boston Area Travel Medicine Network clinics used Focus Diagnostics anti-DENV IgG ELISA to measure anti-DENV IgG antibodies in 591 pretravel specimens from U.S. residents who had traveled to countries where dengue is endemic. When using the manufacturer's index cutoff value for this ELISA, false-positive results were observed that overestimated the perceived past DENV exposure in U.S. travelers. Validation of 121 of these anti-DENV IgG results by plaque reduction neutralization test (PRNT) was used for receiver operating characteristic (ROC) curve optimization of the index cutoff value from 1 to 3.0, improving the specificity of the anti-DENV IgG ELISA from 24% to 95.7%. Additionally, previous vaccination with yellow fever virus contributed to 52.8% of the false-positive rate in the anti-DENV IgG ELISA results. Optimization of the cutoff value of the anti-DENV IgG ELISA provided better interpretation and confidence in the results and eliminated the need for confirmation by PRNT. The travel history of U.S. travelers was also useful for categorizing these travelers into groups for analysis of previous DENV exposure. |
| Databáze: | OpenAIRE |
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