Brown Adipose Tissue Sheds Extracellular Vesicles That Carry Potential Biomarkers of Metabolic and Thermogenesis Activity Which Are Affected by High Fat Diet Intervention
| Autor: | Tamara Camino, Nerea Lago-Baameiro, Aurelio Sueiro, Susana Belén Bravo, Iván Couto, Francisco Fernando Santos, Javier Baltar, Felipe F. Casanueva, María Pardo |
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| Rok vydání: | 2022 |
| Předmět: |
Glucose Transporter Type 1
Proteome Adipose Tissue White Organic Chemistry Ceruloplasmin Thermogenesis General Medicine Diet High-Fat Catalysis Rats Computer Science Applications Rats Sprague-Dawley Inorganic Chemistry Extracellular Vesicles Adipose Tissue Brown Animals obesity extracellular vesicles exosomes adipose tissue white adipose tissue brown adipose tissue batosomes proteome metabolic diseases cell communication Obesity Physical and Theoretical Chemistry Energy Metabolism Molecular Biology Biomarkers Uncoupling Protein 1 Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 18; Pages: 10826 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms231810826 |
| Popis: | Brown adipose tissue (BAT) is a key target for the development of new therapies against obesity due to its role in promoting energy expenditure; BAT secretory capacity is emerging as an important contributor to systemic effects, in which BAT extracellular vesicles (EVs) (i.e., batosomes) might be protagonists. EVs have emerged as a relevant cellular communication system and carriers of disease biomarkers. Therefore, characterization of the protein cargo of batosomes might reveal their potential as biomarkers of the metabolic activity of BAT. In this study, we are the first to isolate batosomes from lean and obese Sprague–Dawley rats, and to establish reference proteome maps. An LC-SWATH/MS analysis was also performed for comparisons with EVs secreted by white adipose tissue (subcutaneous and visceral WAT), and it showed that 60% of proteins were exclusive to BAT EVs. Precisely, batosomes of lean animals contain proteins associated with mitochondria, lipid metabolism, the electron transport chain, and the beta-oxidation pathway, and their protein cargo profile is dramatically affected by high fat diet (HFD) intervention. Thus, in obesity, batosomes are enriched with proteins involved in signal transduction, cell communication, the immune response, inflammation, thermogenesis, and potential obesity biomarkers including UCP1, Glut1, MIF, and ceruloplasmin. In conclusion, the protein cargo of BAT EVs is affected by the metabolic status and contains potential biomarkers of thermogenesis activity. |
| Databáze: | OpenAIRE |
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