Whole genome analysis of the koa wilt pathogen (Fusarium oxysporum f. sp. koae) and the development of molecular tools for early detection and monitoring

Autor:	Tyler C. Jones, Nicklos S. Dudley, R. Kasten Dumroese, Mee-Sook Kim, Aileen Yeh, Robert D. Hauff, Ned B. Klopfenstein, John T. Dobbs, Jane Stewart, P. G. Cannon
Rok vydání:	2020
Předmět:	0106 biological sciences lcsh:QH426-470 lcsh:Biotechnology Koa wilt Virulence PCR primer Biology 01 natural sciences Genome Hawaii Lineage-specific DNA 03 medical and health sciences Fusarium lcsh:TP248.13-248.65 Genetic variation Fusarium oxysporum Genetics medicine Pathogen Gene 030304 developmental biology DNA Primers Plant Diseases 0303 health sciences Acacia koa medicine.disease biology.organism_classification lcsh:Genetics Haplotypes Virulence genes DNA microarray Research Article 010606 plant biology & botany Biotechnology
Zdroj:	BMC Genomics, Vol 21, Iss 1, Pp 1-15 (2020) BMC Genomics
ISSN:	1471-2164
Popis:	Background Development and application of DNA-based methods to distinguish highly virulent isolates of Fusarium oxysporum f. sp. koae [Fo koae; cause of koa wilt disease on Acacia koa (koa)] will help disease management through early detection, enhanced monitoring, and improved disease resistance-breeding programs. Results This study presents whole genome analyses of one highly virulent Fo koae isolate and one non-pathogenic F. oxysporum (Fo) isolate. These analyses allowed for the identification of putative lineage-specific DNA and predicted genes necessary for disease development on koa. Using putative chromosomes and predicted gene comparisons, Fo koae-exclusive, virulence genes were identified. The putative lineage-specific DNA included identified genes encoding products secreted in xylem (e. g., SIX1 and SIX6) that may be necessary for disease development on koa. Unique genes from Fo koae were used to develop pathogen-specific PCR primers. These diagnostic primers allowed target amplification in the characterized highly virulent Fo koae isolates but did not allow product amplification in low-virulence or non-pathogenic isolates of Fo. Thus, primers developed in this study will be useful for early detection and monitoring of highly virulent strains of Fo koae. Isolate verification is also important for disease resistance-breeding programs that require a diverse set of highly virulent Fo koae isolates for their disease-screening assays to develop disease-resistant koa. Conclusions These results provide the framework for understanding the pathogen genes necessary for koa wilt disease and the genetic variation of Fo koae populations across the Hawaiian Islands.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db57a4496abc880903a02eb371c046b3 https://pubmed.ncbi.nlm.nih.gov/33148175 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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