Simple method for plant virus detection: effect of antibody immobilization technique
| Autor: | I.Yu Kostikov, N. S. Dyachenko, Boris A. Snopok, P. M. Boltovets, Yu.M. Shirshov, V. R. Boyko |
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| Rok vydání: | 2002 |
| Předmět: |
Chromatography
Thiocyanate biology Surface Properties Surface Plasmon Resonance Antibodies Viral medicine.disease_cause Microbiology Tobacco Mosaic Virus chemistry.chemical_compound chemistry Chlorophyta Staphylococcus aureus Virology Plant virus biology.protein medicine Gold Gold surface Antibody Surface plasmon resonance Staphylococcal Protein A Protein A Thiocyanates Plant Diseases |
| Zdroj: | Journal of Virological Methods. 105:141-146 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/s0166-0934(02)00098-8 |
| Popis: | The possibility has been demonstrated for applying a surface plasmon resonance for detecting plant viruses in real samples. An optimal mode for antiviral immunoglobulin immobilization on sensor surfaces is described. Out of three proposed techniques for sensor surface treatment, namely, unmodified gold surface, gold surface treated with (a) thiocyanate and (b) thiocyanate and protein A (Staphylococcus aureus), the latter was chosen as most suited for retention of the formed native immunoglobulin layer. |
| Databáze: | OpenAIRE |
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