A microalbuminuria assay using bromphenol blue
| Autor: | Klaus Jung, Erika Nickel, M. Pergande |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Clinical Biochemistry
Buffers Biochemistry Absorbance Phenols Reference Values Albumins medicine Albuminuria Humans Bromphenol Blue Radial immunodiffusion Detection limit Chromatography Chemistry Biochemistry (medical) Albumin General Medicine Hydrogen-Ion Concentration medicine.disease Sephadex Reagent Chromatography Gel Indicators and Reagents Microalbuminuria |
| Zdroj: | Clinica Chimica Acta. 187:163-172 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/0009-8981(90)90343-q |
| Popis: | A simple microalbuminuria assay using bromphenol blue/glycine reagent is described. Urine samples were prepared using gel filtration on Sephadex G-50 minicolumns and absorbance was measured at 610 nm 20 s after mixing 10 parts of eluate and 1 part of reagent. The detection limit of this method was 3 mg/l; within-run and between-run precision was between 0.5 and 4.1% for borderline and raised albumin concentrations. The recovery of albumin added to samples was 98.7 +/- 2.5%. Results obtained by this method correlated closely with values obtained by radial immunodiffusion (r = 0.987). The test is cheap (reagent costs about 5 cents) and suitable for the non-specialist laboratory. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|