Allium cepa root tip assay in assessment of toxicity of magnesium oxide nanoparticles and microparticles
| Autor: | Bhanuramya Mangalampalli, Paramjit Grover, Naresh Dumala |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Environmental Engineering
Mitotic index Metal Nanoparticles chemistry.chemical_element 02 engineering and technology 010501 environmental sciences medicine.disease_cause Plant Roots 01 natural sciences Toxicology Lipid peroxidation chemistry.chemical_compound Onions Toxicity Tests medicine Environmental Chemistry Bioassay Hydrogen peroxide 0105 earth and related environmental sciences General Environmental Science Magnesium General Medicine 021001 nanoscience & nanotechnology Comet assay chemistry Toxicity Biological Assay Lipid Peroxidation Magnesium Oxide 0210 nano-technology Oxidative stress Nuclear chemistry |
| Zdroj: | Journal of Environmental Sciences. 66:125-137 |
| ISSN: | 1001-0742 |
| Popis: | Allium cepa bioassay had been used from decades for the assessment of toxicants and their harmful effects on environment as well as human health. Magnesium oxide (MgO) particles are being utilized in different fields. However, reports on the adverse effects of MgO nanoparticles on the environment and mankind are scarce. Hence, the toxicity of MgO particles is of concern because of their increased utilization. In the current study, A. cepa was used as an indicator to assess the toxicological efficiency of MgO nano- and microparticles (NPs and MPs) at a range of exposure concentrations (12.5, 25, 50, and 100μg/mL). The toxicity was evaluated by using various bioassays on A. cepa root tip cells such as comet assay, oxidative stress and their uptake/internalization profile. Results indicated a dose dependent increase in chromosomal aberrations and decrease in mitotic index (MI) when compared to control cells and the effect was more significant for NPs than MPs (at p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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