Viroporin activity of murine hepatitis virus E protein
| Autor: | Miguel Angel Sanz, Meritxell de Jesús García, Vanessa Madan, Luis Carrasco |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Sindbis virus
Membrane permeability Viral membrane protein viruses Biophysics medicine.disease_cause Biochemistry Permeability Article Virus Mice Viral Proteins chemistry.chemical_compound Structural Biology Cricetinae Escherichia coli Hepatitis E virus Genetics medicine Baby hamster kidney cell Animals SV sindbis virus Coronavirus E protein Molecular Biology Cells Cultured Coronavirus Brefeldin A biology Cell Membrane Membrane Proteins virus diseases Cell Biology IPTG isopropyl-β-d-thio-galactopyranoside biology.organism_classification Virology Anti-Bacterial Agents chemistry Cytoplasm Viroporin activity Hygromycin B MHV murine hepatitis virus |
| Zdroj: | Febs Letters |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/j.febslet.2005.05.046 |
| Popis: | The viroporin activity of the E protein from murine hepatitis virus (MHV), a member of the coronaviruses, was analyzed. Viroporins are a growing family of viral proteins able to enhance membrane permeability, promoting virus budding. Initially, the MHV E gene was inducibly expressed in Escherichia coli cells, leading to the arrest of bacterial growth, cell lysis and permeabilization to different compounds. Thus, exit of labeled nucleotides from E. coli cells to the cytoplasm was apparent upon expression of MHV E. In addition, enhanced entry of the antibiotic hygromycin B occurred at levels comparable to those observed with the viroporin 6K from Sindbis virus. Mammalian cells are also readily permeabilized by the expression of MHV E protein. Finally, brefeldin A powerfully blocks the viroporin activity of the E protein in BHK cells, suggesting that an intact vesicular system is necessary for this coronavirus to permeabilize mammalian cells. |
| Databáze: | OpenAIRE |
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