CAND2/PMTR1 Is Required for Melatonin-Conferred Osmotic Stress Tolerance in Arabidopsis
| Autor: | Yu Zhang, Kai-Kai Lu, Ting-Ting Li, Jia-Xing Guo, Lin-Feng Wang, Wen-Cheng Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Mutant Arabidopsis melatonin 01 natural sciences Receptors G-Protein-Coupled lcsh:Chemistry Gene Expression Regulation Plant Homeostasis lcsh:QH301-705.5 Spectroscopy biology Chemistry ROS General Medicine Catalase Adaptation Physiological Phenotype Up-Regulation Computer Science Applications Cell biology hormones hormone substitutes and hormone antagonists medicine.drug endocrine system Osmotic shock CAND2 Melatonin receptor Article Catalysis Inorganic Chemistry Superoxide dismutase Melatonin 03 medical and health sciences Osmotic Pressure Stress Physiological medicine Physical and Theoretical Chemistry Molecular Biology SNAT1 Arabidopsis Proteins Superoxide Dismutase Organic Chemistry biology.organism_classification 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Seedlings Mutation biology.protein osmotic stress Reactive Oxygen Species 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 4014, p 4014 (2021) International Journal of Molecular Sciences Volume 22 Issue 8 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Osmotic stress severely inhibits plant growth and development, causing huge loss of crop quality and quantity worldwide. Melatonin is an important signaling molecule that generally confers plant increased tolerance to various environmental stresses, however, whether and how melatonin participates in plant osmotic stress response remain elusive. Here, we report that melatonin enhances plant osmotic stress tolerance through increasing ROS-scavenging ability, and melatonin receptor CAND2 plays a key role in melatonin-mediated plant response to osmotic stress. Upon osmotic stress treatment, the expression of melatonin biosynthetic genes including SNAT1, COMT1, and ASMT1 and the accumulation of melatonin are increased in the wild-type plants. The snat1 mutant is defective in osmotic stress-induced melatonin accumulation and thus sensitive to osmotic stress, while exogenous melatonin enhances the tolerance of the wild-type plant and rescues the sensitivity of the snat1 mutant to osmotic stress by upregulating the expression and activity of catalase and superoxide dismutase to repress H2O2 accumulation. Further study showed that the melatonin receptor mutant cand2 exhibits reduced osmotic stress tolerance with increased ROS accumulation, but exogenous melatonin cannot revert its osmotic stress phenotype. Together, our study reveals that CADN2 functions necessarily in melatonin-conferred osmotic stress tolerance by activating ROS-scavenging ability in Arabidopsis. |
| Databáze: | OpenAIRE |
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