Generic benzalkonium chloride-preserved travoprost eye drops are not identical to the branded polyquarternium-1-preserved travoprost eye drop: Effect on cultured human conjunctival goblet cells and their physicochemical properties

Autor: Anne Hedengran, Josefine Clement Freiberg, Pernille May Hansen, Jette Jacobsen, Susan Weng Larsen, Stine Harloff‐Helleberg, Kristine Freude, Gerard Boix‐Lemonche, Goran Petrovski, Steffen Heegaard, Miriam Kolko
Rok vydání: 2022
Předmět:
Zdroj: Hedengran, A, Freiberg, J C, Hansen, P M, Jacobsen, J, Larsen, S W, Harloff-helleberg, S, Freude, K, Boix-lemonche, G, Petrovski, G, Heegaard, S & Kolko, M 2022, ' Generic benzalkonium chloride-preserved travoprost eye drops are not identical to the branded polyquarternium-1-preserved travoprost eye drop : Effect on cultured human conjunctival goblet cells and their physicochemical properties ', Acta Ophthalmologica, vol. 100, no. 7, pp. 819-827 . https://doi.org/10.1111/aos.15163
ISSN: 1755-3768
1755-375X
DOI: 10.1111/aos.15163
Popis: PurposeTo investigate the effect of polyquaternium-1 (PQ)-preserved and benzalkonium chloride (BAK)-preserved travoprost eye drops on viability of primary human conjunctival goblet cell (GC) cultures and on secretion of mucin and cytokines. Furthermore, to evaluate the physicochemical properties of the branded travoprost eye drop Travatan® and available generics.MethodsThe effect of travoprost eye drops was evaluated on GC cultures. Cell viability was assessed through lactate dehydrogenase (LDH) and tetrazolium dye (MTT) colorimetric assays. Mucin secretion was evaluated by immunohistochemical staining. Secretion of interleukin (IL)-6 and IL-8 was measured using BD Cytometric Bead Arrays. pH, viscosity, droplet mass, osmolality and surface tension were measured for all included eye drops.ResultsIn the LDH assay, BAK travoprost caused significant GC loss after 2 hrs of incubation compared to the control. PQ travoprost caused no GC loss at any time point. Both PQ- and BAK travoprost caused secretion of mucin to the cytoplasma. No difference in IL-6 and IL-8 secretion was identified compared to controls. The pH values for the generics were lower (pH 6.0) than the pH value for Travatan (pH 6.7; p ConclusionBAK travoprost caused GC loss, indicating that PQ preservation may be preferable in treatment of glaucoma. Furthermore, physicochemical properties of branded and generic travoprost eye drops can not be assumed to be identical.
Databáze: OpenAIRE
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