SEQUENCE VARIATION OF THE RIBOSOMAL DNA SECOND INTERNAL TRANSCRIBED SPACER REGION IN TWO SPATIALLY DISTINCT POPULATIONS OF AMBLYOMMA AMERICANUM (L.) (ACARI: IXODIDAE)
| Autor: | Mason V. Reichard, A. Alan Kocan, Sidney A. Ewing, R. W. Barker, J. H. Wyckoff, R. A. Van Den Bussche |
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| Rok vydání: | 2005 |
| Předmět: |
Ixodidae
Sequence analysis Molecular Sequence Data Zoology law.invention Amblyomma americanum law DNA Ribosomal Spacer Animals Acari Internal transcribed spacer Ribosomal DNA Phylogeny Ecology Evolution Behavior and Systematics Polymerase chain reaction Polymorphism Genetic Base Sequence biology Phylogenetic tree Genetic Variation biology.organism_classification Molecular biology Female Parasitology Sequence Alignment |
| Zdroj: | Journal of Parasitology. 91:260-263 |
| ISSN: | 1937-2345 0022-3395 |
| Popis: | Sequence analysis of the ribosomal DNA second internal transcribed spacer (ITS 2) region in 2 spatially distinct populations of Amblyomma americanum (L.) revealed intraspecific variation. Nucleotide sequences from multiple DNA extractions and several polymerase chain reaction amplifications of eggs from mixed-parentage samples from both populations of ticks revealed that 12 of 1,145 (1.0%) sites varied. Three of the 12 sites of variation were distinct between the 2 A. americanum populations, which corresponded to a rate of 0.26%. Phylogenetic analysis based on ITS 2 sequences provided strong support (i.e., bootstrap value of 80%) that wild A. americanum clustered into a distinguishable group separate from those derived from colony ticks. |
| Databáze: | OpenAIRE |
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