In vitro efficacy of artemisinin-based treatments against SARS-CoV-2
| Autor: | Santseharay Ramirez, Judith M. Gottwein, Klaus Osterrieder, Long V. Pham, Yuyong Zhou, Eva Settels, Shan Feng, Kerry Gilmore, Ulrik Fahnøe, Jens Bukh, Peter H. Seeberger, Karen A. Gammeltoft, Jakob Trimpert, Anna Offersgaard |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Science 030106 microbiology Artemisia annua Pharmacology Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Chlorocebus aethiops medicine Animals Humans 030212 general & internal medicine Artemether Viability assay Artemisinin Vero Cells EC50 A549 cell Active ingredient Multidisciplinary biology Plant Extracts SARS-CoV-2 Drug discovery business.industry COVID-19 500 Naturwissenschaften und Mathematik::570 Biowissenschaften Biologie::570 Biowissenschaften Biologie biology.organism_classification Artemisinins In vitro COVID-19 Drug Treatment chemistry A549 Cells Viral infection Artesunate Medicine business medicine.drug |
| Zdroj: | Scientific Reports Zhou, Y, Gilmore, K, Ramirez, S, Settels, E, Gammeltoft, K A, Pham, L V, Fahnøe, U, Feng, S, Offersgaard, A, Trimpert, J, Bukh, J, Osterrieder, K, Gottwein, J M & Seeberger, P H 2021, ' In vitro efficacy of artemisinin-based treatments against SARS-CoV-2 ', Scientific Reports, vol. 11, 14571 . https://doi.org/10.1038/s41598-021-93361-y Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) |
| Popis: | Effective and affordable treatments for patients suffering from coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), are needed. We report in vitro efficacy of Artemisia annua extracts as well as artemisinin, artesunate, and artemether against SARS-CoV-2. The latter two are approved active pharmaceutical ingredients of anti-malarial drugs.Proof-of-concept for prophylactic efficacy of the extracts was obtained using a plaque-reduction assay in VeroE6 cells. Subsequent concentration-response studies using a high-throughput antiviral assay, based on immunostaining of SARS-CoV-2 spike glycoprotein, revealed that pretreatment and treatment with extracts, artemisinin, and artesunate inhibited SARS-CoV-2 infection of VeroE6 cells. In treatment assays, artesunate (50% effective concentration (EC50): 7 μg/mL) was more potent than the tested plant extracts (128-260 μg/mL) or artemisinin (151 μg/mL) and artemether (>179 μg/mL), while generally EC50 in pretreatment assays were slightly higher. The selectivity index (SI), calculated based on treatment and cell viability assays, was highest for artemisinin (54), and roughly equal for the extracts (5-10), artesunate (6) and artemether ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|