Short Cold Storage Enhances the Anthocyanin Contents and Level of Transcripts Related to Their Biosynthesis in Blood Oranges
| Autor: | T Crifò, Goffredo Petrone, Angela Roberta Lo Piero, L. Lo Cicero |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Structural gene
food and beverages Cold storage General Chemistry Orange (colour) Biology Anthocyanins Cold Temperature chemistry.chemical_compound Pigment Food Storage chemistry Biosynthesis Gene Expression Regulation Plant Fruit Anthocyanin visual_art Gene expression Botany visual_art.visual_art_medium Food science General Agricultural and Biological Sciences Citrus × sinensis Citrus sinensis Plant Proteins |
| Zdroj: | Journal of Agricultural and Food Chemistry. 60:476-481 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf203891e |
| Popis: | The health benefits associated with the consumption of anthocyanin-containing foods are extensively documented. Mature fruits of blood oranges and their hybrids are characterized by the presence of these bioactive pigments, the abundance of which can be enhanced by storing fruit at cooling nonfreezing temperature. In this work the effects of short low-temperature exposure (4 °C × 15 days) upon orange anthocyanin content and the expression of structural genes belonging to the pigment biosynthesis pathway were investigated. The results highlight that anthocyanin levels of fruit exposed to cold sharply increase, reaching, after 6 days of storage, a value 8 times higher than that observed in the time zero samples, thus suggesting that fruit with enhanced health-related attributes might be obtained at this storage stage. The analysis of gene expression shows that the amount of transcripts of all considered genes (CM1, PAL, CHS, DFR, ANS, UFGT, and GST) sharply increased after 3-6 days of cold storage, confirming previous data showing that the biosynthesis of anthocyanins is a cold-regulated pathway. By comparing the expression of selected genes (PAL, DFR, and UFGT) between blood and common oranges, it turns out that those genes strictly involved in anthocyanin biosynthesis are not cold responsive in common oranges. Moreover, the data highlight that the EST encoding the transcription factor NAC domain protein is selectively induced by cold in blood oranges but not in common oranges, thus proposing it as a candidate gene specifically involved in blood orange response to cold exposure. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|