Surface-Tunable Bioluminescence Resonance Energy Transfer via Geometry-Controlled ZnO Nanorod Coordination
| Autor: | Soo Min Hwang, Young-Pil Kim, Jung Heon Lee, Hansoo Park, Geun Chul Park, Butaek Lim, Jun Hyung Lim, Jinho Joo, Seung Muk Lee, Jung Ho Kim |
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| Rok vydání: | 2015 |
| Předmět: |
Nanostructure
Light Surface Properties Metal ions in aqueous solution chemistry.chemical_element Nanotechnology Geometry Zinc Biomaterials Materials Testing Quantum Dots Fluorescence Resonance Energy Transfer General Materials Science Particle Size Luciferases Renilla Nanotubes Chemistry Resonance General Chemistry Förster resonance energy transfer Quantum dot Luminescent Measurements Nanorod Zinc Oxide Crystallization Biosensor Biotechnology |
| Zdroj: | Small. 11:3469-3475 |
| ISSN: | 1613-6810 |
| DOI: | 10.1002/smll.201403700 |
| Popis: | The use of ZnO nanorods (NRs) as an effective coordinator and biosensing platform to create bioluminescence resonance energy transfer (BRET) is reported. Herein, a hydrothermal approach is applied to obtain morphologically controlled ZnO NRs, which are directly bound to luciferase (Luc) and carboxy-modified quantum dot (QD) acting as a donor-acceptor pair for BRET. BRET efficiency varies significantly with the geometry of ZnO NRs, which modulates the coordination between hexahistidine-tagged Luc (Luc-His6 ) and QD, owing to the combined effect of the total surface area consisting of (001) and (100) planes and their surface polarities. Unlike typical QD-BRET reactions with metal ions (e.g., zinc ions), a geometry-controlled ZnO NR platform can facilitate the design of surface-initiated BRET sensors without being supplemented by copious metal ions: the geometry-controlled ZnO NR platform can therefore pave the way for nanostructure-based biosensors with enhanced analytical performance. |
| Databáze: | OpenAIRE |
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