Long-term culture of human odontoma-derived cells with a Rho kinase inhibitor
| Autor: | Kazuyuki Koike, Yasuyuki Minakawa, Toshikazu Takahara, Morihiro Higo, Masanobu Yamatoji, Dai Nakashima, Atsushi Kasamatsu, Tomoaki Saito, Hideki Tanzawa, Yosuke Sakamoto, Katsuhiro Uzawa, Masashi Shiiba |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Time Factors Pyridines medicine.drug_class Sialoglycoproteins Apoptosis Biology 03 medical and health sciences Calcification Physiologic 0302 clinical medicine Odontoma stomatognathic system Dentin sialophosphoprotein Osteogenesis Tumor Cells Cultured medicine Humans RNA Messenger Protein Kinase Inhibitors Telomerase Cellular Senescence Cell Proliferation Extracellular Matrix Proteins rho-Associated Kinases Telomere Homeostasis Cell Differentiation Cell Biology Protein kinase inhibitor Phosphoproteins medicine.disease Amides Phenotype Coculture Techniques In vitro Telomere Cell biology stomatognathic diseases 030104 developmental biology Biochemistry Rho kinase inhibitor 030220 oncology & carcinogenesis Odontogenesis Stem cell |
| Zdroj: | Experimental Cell Research. 347:232-240 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/j.yexcr.2016.08.005 |
| Popis: | Because of cellular senescence/apoptosis, no effective culture systems are available to maintain replication of cells from odontogenic tumors especially for odontoma, and, thus, the ability to isolate human odontoma-derived cells (hODCs) for functional studies is needed. The current study was undertaken to develop an approach to isolate hODCs and fully characterize the cells in vitro. The hODCs were cultured successfully with a Rho-associated protein kinase inhibitor (Y-27632) for an extended period with stabilized lengths of the telomeres to sustain a similar phenotype/property as the primary tumoral cells. While the hODCs showed stable long-term expansion with expression of major dental epithelial markers including dentin sialophosphoprotein (DSPP) even in the three-dimensional microenvironment, they lack the specific markers for the characteristics of stem cells. Moreover, cells from dental pulp showed significant up-regulation of DSPP when co-cultured with the hODCs, while control fibroblasts with the hODCs did not. Taken together, we propose that the hODCs can be isolated and expanded over the long term with Y-27632 to investigate not only the development of the hODCs but also other types of benign human tumors. |
| Databáze: | OpenAIRE |
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