Effect of cardiolipin on functional properties of isolated rat liver mitochondria
| Autor: | Valentina Bobyleva, Teresa Loredana Pazienza, Monica Bellei, Umberto Muscatello |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
Membrane permeability Cardiolipins Cell Respiration Clinical Biochemistry chemistry.chemical_element Mitochondria Liver Mitochondrion Calcium Biology Biochemistry Membrane Potentials Rats Sprague-Dawley chemistry.chemical_compound Genetics Cardiolipin Animals Inner mitochondrial membrane Receptor Molecular Biology Adenosine Triphosphatases chemistry.chemical_classification ATP/metabolism Calcium/metabolism Cardiolipins/*physiology Cell Respiration Membrane Potentials Mitochondria Liver/*physiology Rats Sprague-Dawley Cell Biology Rats Cell biology Enzyme chemistry Phosphorylation |
| Zdroj: | IUBMB Life. 41:469-480 |
| ISSN: | 1521-6543 |
| DOI: | 10.1080/15216549700201491 |
| Popis: | The specific involvement of cardiolipin in modulating and/or controlling the activity of a number of mitochondrial carriers, enzymes and receptors is well documented; however, comparatively less understood is its role for the integrated functions of intact mitochondria. The aim of the present research was to get a better insight into this problem by investigating the effect of in vitro addition of cardiolipin on the properties of isolated liver mitochondria. The results obtained show that cardiolipin induces extensive structural and functional perturbations at the level of the inner mitochondrial membrane. In fact, addition of cardiolipin to intact mitochondria causes a significant increase of proton leak associated with a parallel increase of respiratory rate in State 4. Concomitantly, a slight uncoupling of phosphorylation associated with a moderate increase in ATPase activity is observed. Furthermore, the pore-mediated membrane permeability to calcium is drastically modified, an effect that can be reversed by addition of cyclosporin. |
| Databáze: | OpenAIRE |
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