SUMO1 in human sperm: new targets, role in motility and morphology and relationship with DNA damage
| Autor: | Paola Piomboni, Sara Marchiani, Gianni Forti, Beatrice Ricci, Daniele Nosi, Elisabetta Baldi, Giuseppe Belmonte, Monica Muratori, Marta Cambi, Lara Tamburrino |
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| Rok vydání: | 2014 |
| Předmět: |
Dynamins
Male endocrine system Embryology DNA damage Somatic cell SUMO-1 Protein SUMO protein Motility DNA Fragmentation Biology Flow cytometry GTP Phosphohydrolases Mitochondrial Proteins Endocrinology Antigens Neoplasm medicine Humans Cell Shape Sperm motility Infertility Male Cryopreservation medicine.diagnostic_test GTPase-Activating Proteins Obstetrics and Gynecology Sumoylation Cell Biology Sperm Spermatozoa Cell biology Mitochondria Cold Temperature DNA-Binding Proteins Oxidative Stress DNA Topoisomerases Type II Reproductive Medicine Sperm Motility DNA fragmentation Sperm Head Microtubule-Associated Proteins DNA Damage Signal Transduction |
| Zdroj: | Reproduction (Cambridge, England). 148(5) |
| ISSN: | 1741-7899 |
| Popis: | In studies carried out previously, we demonstrated that small ubiquitin-like modifier 1 (SUMO1) is associated with poor sperm motility when evaluated with a protocol that reveals mostly SUMO1-ylated live sperm. Recently, with another protocol, it has been demonstrated that SUMO is expressed in most sperm and is related to poor morphology and motility, suggesting that sumoylation may have multiple roles depending on its localisation and targets. We show herein, by confocal microscopy and co-immunoprecipitation, that dynamin-related protein 1 (DRP1), Ran GTPase-activating protein 1 (RanGAP1) and Topoisomerase IIα, SUMO1 targets in somatic and/or germ cells, are SUMO1-ylated in mature human spermatozoa. DRP1 co-localises with SUMO1 in the mid-piece, whereas RanGAP1 and Topoisomerase IIα in the post-acrosomal region of the head. Both SUMO1 expression and co-localisation with the three proteins were significantly higher in morphologically abnormal sperm, suggesting that sumoylation represents a marker of defective sperm. DRP1 sumoylation at the mid-piece level was higher in the sperm of asthenospermic men. As in somatic cells, DRP1 sumoylation is associated with mitochondrial alterations, this protein may represent the link between SUMO and poor motility. As SUMO pathways are involved in responses to DNA damage, another aim of our study was to investigate the relationship between sumoylation and sperm DNA fragmentation (SDF). By flow cytometry, we demonstrated that SUMO1-ylation and SDF are correlated (r=0.4,Pn=37) and most sumoylated sperm shows DNA damage in co-localisation analysis. When SDF was induced by stressful conditions (freezing and thawing and oxidative stress), SUMO1-ylation increased. Following freezing and thawing, SUMO1–Topoisomerase IIα co-localisation and co-immunoprecipitation increased, suggesting an involvement in the formation/repair of DNA breakage. |
| Databáze: | OpenAIRE |
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