Fetal myofibroblast-like cells isolated from post-radiation fibrosis in human breast cancer
| Autor: | Bruno Azzarone, Véronique Magnien, S. BéRanger, A. Tamboise, E. Tamboise, I. Pihan, Lucien Israel, Danièle Brouty-Boyé, H. Raux, L. Zardi |
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| Rok vydání: | 1991 |
| Předmět: |
Cancer Research
Cell type Pathology medicine.medical_specialty Fluorescent Antibody Technique Breast Neoplasms Vimentin Biology Extracellular matrix Fibrosis medicine Humans Fibroblast Chromosome Aberrations Radiotherapy DNA Fibroblasts Middle Aged medicine.disease Fibronectin medicine.anatomical_structure Oncology Cell culture Karyotyping biology.protein Female Myofibroblast Cell Division |
| Zdroj: | International Journal of Cancer. 47:697-702 |
| ISSN: | 1097-0215 0020-7136 |
| Popis: | Cells were isolated from post-radiation fibrosis biopsies of patients with recurrent breast carcinoma. These cells were identified as fibroblasts and compared with fibroblasts from normal breast tissues for their proliferative activities, chromosome number and for the presence of various components of the extracellular matrix and cytoskeleton. The proliferative activity of the fibrosis-derived fibroblasts did not significantly differ from that of normal breast fibroblasts. Both cell types required serum to grow and did not form colonies in soft agar. Cells from 2 of the 3 fibroses analyzed displayed aneuploid karyotypes with multiple structural abnormalities. All of the fibroblastic cells produced types I, III and V collagen, fibronectin and vimentin. However, in contrast to normal breast fibroblasts, fibrosis-derived cells produced high amounts of oncofetal fibronectin. In addition, fibrosis of fibroblasts also expressed the alpha-actin isoform which is specific for smooth-muscle cells. These results suggest that post-radiation fibrosis in malignant breast contains atypical fibroblasts with fetal and myofibroblastic characteristics. |
| Databáze: | OpenAIRE |
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