Role of proteoglycans on the biochemical and biomechanical properties of dentin organic matrix
| Autor: | Momina Rahman, Ana K. Bedran-Russo, Ariene A. Leme-Kraus, Cristina de Mattos Pimenta Vidal, Ana Paula Farina |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Compressive Strength Dentistry In Vitro Techniques Matrix (biology) Collagen Type I Article Extracellular matrix Glycosaminoglycan 03 medical and health sciences 0302 clinical medicine Microscopy Electron Transmission stomatognathic system Tensile Strength Ultimate tensile strength medicine Dentin Humans Tooth Demineralization General Dentistry Glycosaminoglycans Chemistry business.industry 030206 dentistry Cell Biology General Medicine Trypsin Molar Biomechanical Phenomena Extracellular Matrix 030104 developmental biology medicine.anatomical_structure Otorhinolaryngology Biophysics Anisotropy Proteoglycans business Type I collagen Biomineralization medicine.drug |
| Zdroj: | Archives of Oral Biology. 82:203-208 |
| ISSN: | 0003-9969 |
| Popis: | Objective Proteoglycans (PGs) are multifunctional biomacromolecules of the extracellular matrix of collagen-based tissues. In teeth, besides a pivotal regulatory role on dentin biomineralization, PGs provide mechanical support to the mineralized tissue and compressive strength to the biosystem. This study assessed enzymatic protocols for selective PGs removal from demineralized dentin to determine the roles of these biomacromolecules in the bulk mechanical properties and biostability of type I collagen. Methods Selective removal of glycosaminoglycans chains (GAGs) and PGs from demineralized dentin was carried out by enzymatic digestion protocols using chondroitinase ABC (c-ABC) and trypsin (Try). A comprehensive study design included assessment of dentin matrix mass loss, biodegradability of the PGs/GAGs-depleted dentin matrix, ultimate tensile strength (UTS) and energy to fracture tests. Quantitative data was statistically analyzed by two-way and one-way ANOVA followed by the appropriate post hoc tests (α = 0.05). Results Transmission electron microscopy images show effective GAGs removal by c-ABC and Try and both enzymatic methods released statistically similar amounts of GAGs from the demineralized dentin. Try digestion resulted in about 25% dentin matrix mass loss and increased susceptibility to collagenolytic digestion when compared to c-ABC (p = 0.0224) and control (p = 0.0901). Moreover, PGs digestion by Try decreased the tensile strengths of dentin. Statistically lower energy to fracture was observed in c-ABC-treated dentin matrix. Conclusions GAGs plays a pivotal role on tissue mechanics and anisotropy, while the core protein of PGs have a protective role on matrix biostability. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect