Tattoo pigment agglomerates measured in skin biopsies by computerised light microscopy
| Autor: | Katrina Hutton Carlsen, Georg Larsen, Jørgen Serup |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Microscopy
medicine.medical_specialty Sarcoidosis Tattooing business.industry Biopsy Dermatology Skin Diseases 01 natural sciences 010309 optics 030207 dermatology & venereal diseases 03 medical and health sciences symbols.namesake 0302 clinical medicine 0103 physical sciences symbols Humans Medicine In patient Tattoo pigment Coloring Agents business Fisher's exact test Skin |
| Zdroj: | Skin Research and Technology. 26:284-291 |
| ISSN: | 1600-0846 0909-752X |
| Popis: | Background Black tattoo reactions are suggested to be foreign body reactions manifested as papulo-nodular inflammation that is associated with active sarcoidosis. Aim To study the morphology of black and red pigment agglomerates in skin biopsies from tattoo reactions, referenced to clinical diagnoses. Method Agglomerate count, area, width, height, circumference and circularity were measured in 161 patients by light microscopy (Olympus BX51™); 161 unstained skin biopsies from 64 black tattoo reactions and 97 reactions in red tattoos with allergy. Images (Jenoptic Gryphax RGB camera) were taken through a 40× objective using immersion oil. Computerised analysis of agglomerates was performed using ImageJ software. Student t test, chi-square test, Fisher Exact test, ANOVA and Bonferroni tests were applied. Results Comparison of black and red pigment agglomerates showed no overall differences in count, area, width, height or circumference. However, in black tattoo reactions, the count of agglomerates was higher in patients with sarcoidosis (P = .009) vs no sarcoidosis. Black agglomerates were more circular shaped as compared to red, P = .023. In red tattoo reactions, agglomerates in patients with allergic cross-reactivity were slightly more circular (P = .036) vs patients with milder allergy. The count of red agglomerates was lower in patients clinically typed excessive hyperkeratosis (P = .041) compared with other clinical types of allergy. Conclusion Patients with reactions in black tattoos associated with sarcoidosis have increased count of pigment agglomerates vs those without associated sarcoidosis indicating that black pigment agglomeration is one among a number of factors triggering sarcoid tattoo complication. Circular shape of agglomerates may code for bioactivity. |
| Databáze: | OpenAIRE |
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