Prevalence, geographic risk factor, and development of a standardized protocol for fungal isolation in cystic fibrosis: Results from the international prospective study 'MFIP'

Autor: Laurence Delhaes, Florent Morio, Wieland Meyer, Jean-Philippe Bouchara, A. Biffi, E. Manso, Gabriella Ricciotti, Rafael Cantón, Patrice Le Pape, Gilles Nevez, Maiz-Luis Carro, Kada Touati, Walter Buzina, Lisa Cariani, Ersilia Fiscarelli, Marc Pihet, Elia Gómez, Frédéric Grenouillet, Françoise Botterel, Veroniek Saegeman, Muriel Cornet, Alain Duhamel, Silvia Campana, Solène Le Gal, Katrien Lagrou, Odile Faure-Cognet, Eric Dannaoui, Sharon C.-A. Chen, Andrew M. Borman, Emmanuel Roilides, Loïc Favennec, Maria Simitsopoulou
Přispěvatelé: Thérapeutique Recombinante Expérimentale (TIMC-IMAG-TheREx), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Hospital Universitario Ramón y Cajal [Madrid], Universidad de Alcalá - University of Alcalá (UAH), Laboratoire de Parasitologie-Mycologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Centre hospitalier universitaire de Nantes (CHU Nantes), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Université de Reims Champagne-Ardenne (URCA), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Epidémiosurveillance de protozooses à transmission alimentaire et vectorielle (ESCAPE), Université de Reims Champagne-Ardenne (URCA)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), Laboratoire de Parasitologie et Mycologiede [CHRU Brest], Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Mycology Reference Laboratory, Health Protection Agency, Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), The University of Sydney, Third Department of Pediatrics, Aristotle University, Ippokration Hospital of Thessaloniki, Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, Children's Hospital Bambino Gesù IRCCS [Rome]
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Male
Microbiological Techniques
0301 basic medicine
Cystic Fibrosis
International Cooperation
[SDV]Life Sciences [q-bio]
Cystic fibrosis
Aspergillus fumigatus
Molds
0302 clinical medicine
Risk Factors
Prevalence
Prospective Studies
Prospective cohort study
ComputingMilieux_MISCELLANEOUS
education.field_of_study
biology
3. Good health
Aspergillus
Female
medicine.symptom
Cladosporium
Adult
Pulmonary and Respiratory Medicine
medicine.medical_specialty
Filamentous fungi
Lung mycobiota
Population
Context (language use)
Specimen Handling
03 medical and health sciences
Internal medicine
Mycological examination
medicine
Humans
education
Lung Diseases
Fungal
business.industry
Fungi
Sputum
Reproducibility of Results
medicine.disease
biology.organism_classification
Culture Media
030104 developmental biology
030228 respiratory system
Pediatrics
Perinatology and Child Health
business
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Zdroj: Journal of Cystic Fibrosis
Journal of Cystic Fibrosis, Elsevier, 2019, 18 (2), pp.212-220. ⟨10.1016/j.jcf.2018.10.001⟩
ISSN: 1569-1993
DOI: 10.1016/j.jcf.2018.10.001⟩
Popis: © 2018 Background: Fungi are increasingly recognized for their potential role in contributing to pulmonary damage in Cystic Fibrosis (CF). We therefore designed a prospective international study aimed at (i) determining the prevalence of fungi isolated from sputum samples collected from a large CF population, (ii) comparing the performance of different media used for fungal culture, and (iii) proposing a standardized protocol suitable for CF routine microbiology. Methods: An international, consensually designed prospective study was set up (https://www.ecfs.eu/special-projects/mucofong-international-project). All centers worked according to the same protocol approved by Lille Ethical Committee. CF sputa were inoculated onto eight semi-selective media incubated at 37 °C or 25 °C–30 °C for 15 days, and inspected twice weekly for fungal growth. Results: A total of 469 sputa were collected from patients at 18 European and one Australian CF centers. Positive cultures for fungal growth were significantly associated with patient ages. Aspergillus fumigatus was the most frequently isolated mold. We identified a growing European North-to-South gradient of Scedosporium prevalence, while yeasts, Aspergillus section Fumigati, Cladosporium and Penicillium were significantly more prevalent in the Northern regions. Conclusions: According to the CHi-squared Automatic Interaction Detector method, we propose a consensual protocol based on two media (YPDA or Sabouraud medium, and B(+) medium) to detect the main opportunistic molds in CF context; the use of an additional medium being recommended according to the patient's clinical status. This standardized protocol allows us to have an accurate overview of the respiratory mycobiome on the culturomic side in CF. ispartof: JOURNAL OF CYSTIC FIBROSIS vol:18 issue:2 pages:212-220 ispartof: location:Netherlands status: published
Databáze: OpenAIRE
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