Transcription Profiles of Endothelial Cells in the Rat Ductus Arteriosus during a Perinatal Period
| Autor: | Susumu Minamisawa, Takashi Kato, Ping Lü, Tomohiro Yokota, Inbun Tei, Norika Mengchia Liu, Utako Yokoyama, Shun Maekawa, Hideki Taniguchi |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
TBX1
HOXA4 Transcription Genetic Endothelium Morphogenesis lcsh:Medicine Biology Real-Time Polymerase Chain Reaction Pregnancy Gene expression medicine Animals Rats Wistar lcsh:Science DNA Primers Oligonucleotide Array Sequence Analysis Genetics Multidisciplinary Base Sequence Cluster of differentiation Gene Expression Profiling lcsh:R Ductus Arteriosus Rats Cell biology Gene expression profiling Vascular endothelial growth factor A medicine.anatomical_structure Female lcsh:Q Endothelium Vascular Research Article |
| Zdroj: | PLoS ONE, Vol 8, Iss 9, p e73685 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0073685 |
| Popis: | Endothelial cells (ECs) lining the blood vessels serve a variety of functions and play a central role in the homeostasis of the circulatory system. Since the ductus arteriosus (DA) has different arterial characteristics from its connecting vessels, we hypothesized that ECs of the DA exhibited a unique gene profile involved in the regulation of DA-specific morphology and function. Using a fluorescence-activated cell sorter, we isolated ECs from pooled tissues from the DA or the descending aorta of Wistar rat fetuses at full-term of gestation (F group) or neonates 30 minutes after birth (N group). Using anti-CD31 and anti-CD45 antibodies as cell surface markers for ECs and hematopoietic derived cells, respectively, cDNAs from the CD31-positive and CD45-negative cells were hybridized to the Affymetrix GeneChip® Rat Gene 1.0 ST Array. Among 26,469 gene-level probe sets, 82 genes in the F group and 81 genes in the N group were expressed at higher levels in DA ECs than in aortic ECs (p2.0). In addition to well-known endothelium-enriched genes such as Tgfb2 and Vegfa, novel DA endothelium-dominant genes including Slc38a1, Capn6, and Lrat were discovered. Enrichment analysis using GeneGo MetaCore software showed that DA endothelium-related biological processes were involved in morphogenesis and development. We identified many overlapping genes in each process including neural crest-related genes (Hoxa1, Hoxa4, and Hand2, etc) and the second heart field-related genes (Tbx1, Isl1, and Fgf10, etc). Moreover, we found that regulation of epithelial-to-mesenchymal transition, cell adhesion, and retinol metabolism are the active pathways involved in the network via potential interactions with many of the identified genes to form DA-specific endothelia. In conclusion, the present study uncovered several significant differences of the transcriptional profile between the DA and aortic ECs. Newly identified DA endothelium-dominant genes may play an important role in DA-specific functional and morphologic characteristics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|