Characterization of Prostanoid Receptors in Podocytes
| Autor: | Rolf M. Nüsing, Martin J. Bek, Pascal Kowark, Anna Henger, Peter Mundel, Hermann Pavenstädt |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Calcium Isotopes
Agonist medicine.medical_specialty Patch-Clamp Techniques medicine.drug_class Prostaglandin E2 receptor Kidney Glomerulus Molecular Sequence Data Radioimmunoassay EP4 Receptor Prostaglandin Dinoprost Polymerase Chain Reaction Sensitivity and Specificity Dinoprostone Podocyte Mice chemistry.chemical_compound Cell surface receptor Internal medicine Cyclic AMP medicine Animals Receptors Prostaglandin E Endothelium RNA Messenger Prostaglandin E2 Receptor Cells Cultured Ion Transport Base Sequence Chemistry Cell Membrane General Medicine medicine.anatomical_structure Endocrinology Nephrology 15-Hydroxy-11 alpha 9 alpha-(epoxymethano)prosta-5 13-dienoic Acid medicine.drug |
| Zdroj: | Journal of the American Society of Nephrology. 10:2084-2093 |
| ISSN: | 1046-6673 |
| DOI: | 10.1681/asn.v10102084 |
| Popis: | Prostaglandins participate in the regulation of important glomerular functions and are involved in the pathogenesis of glomerular diseases. This study investigates the influence of prostaglandins on membrane voltage, ion conductances, cAMP accumulation, and cytosolic calcium activity ([Ca2+]i) in differentiated podocytes. Prostaglandin E2 (PGE2) caused a concentration-dependent depolarization and an increase of the whole cell conductance in podocytes (EC50 approximately 50 nM). Compared with PGE2, the EP2/EP3/EP4 receptor agonist 11-deoxy-PGE1 caused an equipotent depolarization, whereas the DP receptor agonist BW 245 C, the EP1/EP3 receptor agonist sulprostone, and the IP receptor agonist iloprost were at least 100 to 1000 times less potent than PGE2. The EP2 receptor agonist butaprost did not change membrane voltage of podocytes. The depolarizing effect of PGE2 was increased in an extracellular solution with a reduced Cl- concentration (from 145 to 32 mM). PGE2 and the prostaglandin agonists, but not the IP receptor agonist iloprost and the EP2 receptor agonist butaprost, induced a time- and concentration-dependent cAMP accumulation in podocytes. In fura-2 fluorescence experiments, PGE2, sulprostone, PGF2alpha, fluprostenol (a potent FP agonist), and U-46619 (a selective thromboxane A2 agonist) induced a biphasic increase of [Ca2+]i in 60 to 80% of podocytes. In reverse transcription-PCR studies, podocyte mRNA for the EP1, EP4, FP, and TP receptor could be amplified. These data indicate that in podocytes, PGE2 regulates distinct cellular functions via the EP1 and EP4 receptor, thereby increasing [Ca2+]i and cAMP, respectively. Furthermore, PGF1alpha and U-46619 increase [Ca2+]i via their specific receptors. |
| Databáze: | OpenAIRE |
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