Electrochemical detection of redox molecules secreted by Pseudomonas aeruginosa – Part 1: Electrochemical signatures of different strains

Autor: Pascal Mailley, Sylvie Elsen, Thibaut Cohu, George G. Malliaras, Julie Oziat, Maxime Gougis
Přispěvatelé: Département Bioélectronique (BEL-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-CMP-GC, Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Groupe Pathogenèse Bactérienne et Réponses Cellulaires / Bacterial Pathogenesis and Cellular Responses Group (IBS-PBRC), Institut de biologie structurale (IBS - UMR 5075), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)
Rok vydání: 2021
Předmět:
MESH: Oxidation-Reduction
Biophysics
02 engineering and technology
Quinolones
Bacterial growth
medicine.disease_cause
Electrochemistry
01 natural sciences
Redox
chemistry.chemical_compound
Pyocyanin
Electrochemical signature
medicine
Humans
Pseudomonas Infections
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
Physical and Theoretical Chemistry
MESH: Humans
MESH: Quinolones
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Structural Biology [q-bio.BM]
biology
Strain (chemistry)
Pseudomonas aeruginosa
Chemistry
010401 analytical chemistry
MESH: Pseudomonas Infections
Electrochemical Techniques
General Medicine
021001 nanoscience & nanotechnology
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
MESH: Electrochemical Techniques
0104 chemical sciences
Pseudomonas Quinolone Signal
MESH: Pseudomonas aeruginosa
Pyocyanine
Autoinducer
0210 nano-technology
Oxidation-Reduction
MESH: Pyocyanine
Bacteria
Zdroj: Bioelectrochemistry
Bioelectrochemistry, 2021, 140, pp.107747. ⟨10.1016/j.bioelechem.2021.107747⟩
Bioelectrochemistry, Elsevier, 2021, 140, pp.107747. ⟨10.1016/j.bioelechem.2021.107747⟩
ISSN: 1567-5394
Popis: International audience; During infections, fast identification of the microorganisms is critical to improve patient treatment and to better manage antibiotics use. Electrochemistry exhibits several advantages for rapid diagnostic: it enables easy, cheap and in situ analysis of redox molecules in most liquids. In this work, several culture supernatants of different Pseudomonas aeruginosa strains (including PAO1 and its isogenic mutants PAO1ΔpqsA, PA14, PAK and CHA) were analyzed by square wave voltammetry on glassy carbon electrode during the bacterial growth. The obtained voltamograms shown complex traces exhibiting numerous redox peaks with potential repartitions and current amplitudes depending on the studied bacterium and/or growth time. Among them, some peaks were clearly associated to the well-known redox toxin Pyocyanin (PYO) and the autoinducer Pseudomonas Quinolone Signal (PQS). Other peaks were observed that are not yet attributed to known secreted species. Each complex electrochemical response (number of peaks, peak potential and amplitude) can be interpreted as a fingerprint or "ID-card" of the studied strain that may be implemented for fast bacteria strain identification.
Databáze: OpenAIRE
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