Genome-wide DNA methylation analysis in renal ischemia reperfusion injury
| Autor: | Puxun Tian, Yanlong Zhao, Wujun Xue, Jin Zheng, Xiaoming Ding, Xinxin Xia, Yi Gao, Sutong Li, Feng Zhu, Jing Liu, Feng Han, Chenguang Ding |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Bisulfite sequencing 030232 urology & nephrology Biology Kidney urologic and male genital diseases Dioxygenases Mice 03 medical and health sciences 0302 clinical medicine Databases Genetic Genetics medicine Animals Epigenetics Promoter Regions Genetic DNA Modification Methylases Promoter General Medicine Methylation DNA Methylation Molecular biology Gene Ontology 030104 developmental biology medicine.anatomical_structure CpG site Reperfusion Injury Reduced representation bisulfite sequencing DNA methylation |
| Zdroj: | Gene. 610:32-43 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/j.gene.2017.02.005 |
| Popis: | Renal ischemia reperfusion injury (IRI) is frequently encountered after kidney transplantation and is a leading cause of acute renal failure. Aberrant gene expression and epigenetic regulation occur during the pathophysiology of IRI. In this study, we used reduced representation bisulfite sequencing to identify the DNA methylome of renal tissues during IRI and the sham-operated tissues in C57BL/6. The methylation status of approximately 1.29 million CpGs located in an average of 11554 CpG islands and 17113 promoters in genome was determined. Compared with sham-operated kidney, both acute and chronic IRI significantly decreased the genome-wide methylation level (1.1-1.8%) and the CpG methylation level in the promoter (0.4-0.5%), CpG island (0.5-1.3%), exon (1.3-1.9%), and intron (0.8-1.1%; all P |
| Databáze: | OpenAIRE |
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