Two Clusters of Fluoroquinolone and Clindamycin-ResistantClostridium difficilePCR Ribotype 001 Strain Recognized by Capillary Electrophoresis Ribotyping and Multilocus Variable Tandem Repeat Analysis

Autor: Ed J. Kuijper, Otakar Nyc, Luboš Kubíček, Marcela Krutova, Jana Matejkova, Kríz Z, Filip Ruzicka, Renata Tejkalová
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
Moxifloxacin
Gene Expression
Minisatellite Repeats
Ribotyping
Tertiary Care Centers
0302 clinical medicine
Ciprofloxacin
Drug Resistance
Multiple
Bacterial
030212 general & internal medicine
Czech Republic
antimicrobial drug resistance
Clindamycin
Clostridium difficile
Anti-Bacterial Agents
3. Good health
DNA Gyrase
Vancomycin
Female
Fluoroquinolones
medicine.drug
DNA
Bacterial
Microbiology (medical)
030106 microbiology
Immunology
Microbial Sensitivity Tests
Multiple Loci VNTR Analysis
Biology
Microbiology
03 medical and health sciences
PCR ribotype 001
Metronidazole
medicine
Humans
Aged
Retrospective Studies
Pharmacology
Thr82Ile
Clostridioides difficile
MLVA
Electrophoresis
Capillary
Methyltransferases
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
Virology
capillary electrophoresis ribotyping
Amino Acid Substitution
Clostridium Infections
Multilocus sequence typing
Multilocus Sequence Typing
Zdroj: Microbial Drug Resistance, 23(5), 609-615
ISSN: 1931-8448
1076-6294
DOI: 10.1089/mdr.2016.0159
Popis: To perform a retrospective analysis of the high occurrence of Clostridium difficile infection in the surgical department of a Czech tertiary care hospital and to identify weaknesses in C. difficile infection (CDI) prevention and control policies.Clinical and epidemiological data on eleven CDI cases were collected. C. difficile isolates were characterized by capillary electrophoresis ribotyping, multilocus variable tandem repeat analysis (MLVA), gyrA gene fragment sequencing, and erm(B) fragment PCR amplification. Antibiotic susceptibility to metronidazole, vancomycin, ciprofloxacin, moxifloxacin, and clindamycin was tested.Eleven CDI cases were caused by C. difficile PCR ribotype 001 strains. These strains revealed two different MLVA profiles with 11 tandem repeat differences. All isolates were susceptible to metronidazole and vancomycin and resistant to ciprofloxacin (MIC ≥32 mg/L), moxifloxacin (MIC ≥32 mg/L), and clindamycin (MIC ≥256 mg/L). All isolates revealed amino acid substitution Thr82Ile, in the GyrA and were erm(B) negative.Two fluoroquinolone and clindamycin-resistant C. difficile PCR ribotype 001 strain clusters occurred at one of the surgical departments of a tertiary care hospital. Ineffective decontamination with suboptimal concentration and time of exposure of sporicidal disinfectants may have resulted in C. difficile transmission.
Databáze: OpenAIRE
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