Localization of Interleukin-6 Receptor mRNA in the Pregnant and Non-pregnant Mouse Uterus
| Autor: | Eiichi Hondo, Kahori Kato, Keiji Kokubu, Yasuo Kiso |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Stromal cell Mice Inbred Strains In situ hybridization Biology Mice Stroma Pregnancy Internal medicine Cytokine Receptor gp130 medicine Animals Embryo Implantation RNA Messenger Receptor Interleukin 6 In Situ Hybridization Messenger RNA Interleukin-6 Uterus medicine.disease Endocrinology Interleukin-6 receptor biology.protein Pregnancy Animal Female Animal Science and Zoology Stromal Cells Signal Transduction |
| Zdroj: | Journal of Reproduction and Development. 51:777-781 |
| ISSN: | 1348-4400 0916-8818 |
| DOI: | 10.1262/jrd.17042 |
| Popis: | To understand roles of interleukin 6 (IL-6) family cytokines for pregnancy in mice, localization of IL-6 receptor (IL-6R) mRNA was investigated in non- and early pregnant uteri by in situ hybridization. IL-6R mRNA was expressed in all non-pregnant uteri and in pregnant uteri from the third day (Day 3) to the sixth day of pregnancy (Day 6; the day of plug = Day 1). IL-6R mRNA signals were detected in non-pregnant mice in the luminal and glandular epithelium. Signal strength varied according to the sexual cycle. There was no correlation between the signal strength of the IL-6R mRNA and the serum concentrations of progesterone and 17beta-estradiol, which show a monophasic rise in the non-pregnant sexual cycle. In pregnant mice, slight signals were detectable in the luminal and glandular epithelium on Day 3. IL-6R mRNA messages increased with progression towards Day 4, however, localization changed drastically on Day 5. Stromal cells abruptly expressed their mRNA on Day 5, and these cells strongly expressed it on Day 6. The function of IL-6R in the luminal and glandular epithelium might be different from that in the stroma during the implantation period. In addition, few signals were identified in the stromal cells adjacent to the luminal epithelium on Day 6. This suggests that there are two types of stromal cells on Day 6 in mice. |
| Databáze: | OpenAIRE |
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